Detailed information of TA system TA059676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1649848..1650262
• Replicon: chromosome
• Accession: NZ_CP013953
• Organism: Staphylococcus aureus strain NCCP14558 isolate sequencing

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ASL16_RS08095
• Protein ID: WP_031267622.1
• Coordinates: 1650011..1650262 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASL16_RS16220
• Protein ID: WP_000028424.1
• Coordinates: 1649848..1650021 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASL16_RS08060	1645112..1645402	-	291	WP_000665205.1	VRR-NUC domain-containing protein	-
ASL16_RS08065	1645536..1645634	+	99	WP_001801650.1	hypothetical protein	-
ASL16_RS08070	1645743..1648190	-	2448	WP_000884870.1	virulence-associated E family protein	-
ASL16_RS08075	1648478..1648678	-	201	WP_000265252.1	DUF1514 family protein	-
ASL16_RS08080	1648746..1648898	-	153	WP_000595263.1	transcriptional activator RinB	-
ASL16_RS08085	1648895..1649101	-	207	WP_000195810.1	DUF1381 domain-containing protein	-
ASL16_RS08090	1649138..1649671	-	534	WP_063653166.1	dUTP diphosphatase	-
ASL16_RS16215	1649686..1649847	-	162	WP_000889684.1	hypothetical protein	-
ASL16_RS16220	1649848..1650021	-	174	WP_000028424.1	hypothetical protein	Antitoxin
ASL16_RS08095	1650011..1650262	-	252	WP_031267622.1	DUF1024 family protein	Toxin
ASL16_RS08100	1650255..1650562	-	308	Protein_1559	hypothetical protein	-
ASL16_RS08105	1650559..1650906	-	348	WP_000979209.1	hypothetical protein	-
ASL16_RS08110	1650903..1651283	-	381	WP_001668982.1	hypothetical protein	-
ASL16_RS08115	1651286..1651492	-	207	WP_000693989.1	hypothetical protein	-
ASL16_RS08120	1651506..1651754	-	249	WP_060474161.1	hypothetical protein	-
ASL16_RS08125	1651754..1652008	-	255	WP_000022722.1	DUF3310 domain-containing protein	-
ASL16_RS08130	1652005..1652409	-	405	WP_044426014.1	phage DNA polymerase	-
ASL16_RS08135	1652409..1652594	-	186	WP_001164629.1	DUF3113 family protein	-
ASL16_RS08140	1652607..1654559	-	1953	WP_078064460.1	DNA polymerase	-
ASL16_RS08145	1654627..1655184	-	558	WP_000645045.1	DUF2815 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1614816..1681149	66333

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9419.35 Da
• Isoelectric Point: 3.9476

>T59676 WP_031267622.1 NZ_CP013953:c1650262-1650011 [Staphylococcus aureus]
MNNREKIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGIELDEAVGIMTGQVVYKYEEEQE
NEY

Nucleotide

• Length: 252 bp

>T59676 NZ_CP013953:c1650262-1650011 [Staphylococcus aureus]
ATGAATAATCGCGAAAAAATCGAACAGTCCGTTATTAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTGCTAAA
AGAGATTGAGGACGTGTATAAGAAAGCACAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTATTGAACTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6468.29 Da
• Isoelectric Point: 4.2484

>AT59676 WP_000028424.1 NZ_CP013953:c1650021-1649848 [Staphylococcus aureus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIIDFITKPIYLIKGDE

Nucleotide

• Length: 174 bp

>AT59676 NZ_CP013953:c1650021-1649848 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTAA