Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3796522..3797024 Replicon chromosome
Accession NZ_CP013952
Organism Escherichia coli strain HST04

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AWH44_RS19445 Protein ID WP_000767829.1
Coordinates 3796770..3797024 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AWH44_RS19440 Protein ID WP_001259255.1
Coordinates 3796522..3796773 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWH44_RS19410 3791700..3792767 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AWH44_RS19415 3792767..3793837 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
AWH44_RS19420 3793834..3795138 - 1305 WP_000009594.1 histidinol dehydrogenase -
AWH44_RS19425 3795144..3796043 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
AWH44_RS19435 3796189..3796239 - 51 WP_001364200.1 his operon leader peptide -
AWH44_RS19440 3796522..3796773 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AWH44_RS19445 3796770..3797024 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AWH44_RS19450 3797107..3797931 + 825 WP_000754737.1 SDR family oxidoreductase -
AWH44_RS19455 3797977..3798906 + 930 WP_000803351.1 LysR family transcriptional regulator -
AWH44_RS23905 3799121..3799183 + 63 WP_010723108.1 membrane protein YoeI -
AWH44_RS19460 3799173..3800531 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AWH44_RS19465 3800710..3801768 + 1059 WP_000492339.1 transport protein YeeE -
AWH44_RS19470 3801782..3802009 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T59661 WP_000767829.1 NZ_CP013952:3796770-3797024 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T59661 NZ_CP013952:3796770-3797024 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT59661 WP_001259255.1 NZ_CP013952:3796522-3796773 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT59661 NZ_CP013952:3796522-3796773 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References