Detailed information of TA system TA059642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4846029..4846639
• Replicon: chromosome
• Accession: NZ_CP013949
• Organism: Bradyrhizobium sp. CCGE-LA001

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A0X8CJV5
• Locus tag: BCCGELA001_RS22575
• Protein ID: WP_008549185.1
• Coordinates: 4846355..4846639 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BCCGELA001_RS22570
• Protein ID: WP_008549183.1
• Coordinates: 4846029..4846346 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCCGELA001_RS36110	4841586..4842008	-	423	WP_083543520.1	hypothetical protein	-
BCCGELA001_RS22550	4842449..4843440	+	992	Protein_4531	glycosyltransferase	-
BCCGELA001_RS22555	4843643..4844437	-	795	WP_060736359.1	ATPase	-
BCCGELA001_RS22560	4844490..4844789	-	300	WP_008566478.1	hypothetical protein	-
BCCGELA001_RS22565	4844830..4846032	-	1203	WP_060736360.1	RluA family pseudouridine synthase	-
BCCGELA001_RS22570	4846029..4846346	-	318	WP_008549183.1	HigA family addiction module antidote protein	Antitoxin
BCCGELA001_RS22575	4846355..4846639	-	285	WP_008549185.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BCCGELA001_RS22580	4846696..4848030	-	1335	WP_008549186.1	replication-associated recombination protein A	-
BCCGELA001_RS22585	4848027..4849433	-	1407	WP_060736361.1	DegQ family serine endoprotease	-
BCCGELA001_RS22590	4849746..4851359	+	1614	WP_060736362.1	OprO/OprP family phosphate-selective porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (9-66)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10565.72 Da
• Isoelectric Point: 7.1661

>T59642 WP_008549185.1 NZ_CP013949:c4846639-4846355 [Bradyrhizobium sp. CCGE-LA001]
MIVSYRDKRTERFAAGQHVKEFSGFARQAETRLDRLDAATSLADLAALPGNRQEASKGNRAGQFSIRINDQWRICSNWPE
GSLGPTDVEIVDYH

Nucleotide

• Length: 285 bp

>T59642 NZ_CP013949:c4846639-4846355 [Bradyrhizobium sp. CCGE-LA001]
ATGATCGTCAGCTATCGCGACAAGCGCACCGAGCGATTTGCCGCTGGTCAGCACGTCAAGGAATTCTCGGGATTTGCCCG
CCAGGCAGAGACGCGCCTTGATCGGTTGGATGCGGCAACGAGCTTAGCTGACTTGGCGGCATTGCCCGGCAATCGGCAGG
AAGCCTCGAAGGGCAATCGTGCAGGACAATTCTCGATCCGGATCAACGATCAATGGCGGATTTGCTCCAATTGGCCAGAG
GGATCCCTTGGACCAACTGACGTAGAGATCGTCGACTATCACTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11686.34 Da
• Isoelectric Point: 10.5812

>AT59642 WP_008549183.1 NZ_CP013949:c4846346-4846029 [Bradyrhizobium sp. CCGE-LA001]
MPRTPIHPGEQLAEELRELGITAAELARQIDVPVNRVTGIINGQRGITADTALRLGHWFGTSPQFWMNLQQQYELRLAEK
EIGAQVASLPRRTNAQSTRKLGKTA

Nucleotide

• Length: 318 bp

>AT59642 NZ_CP013949:c4846346-4846029 [Bradyrhizobium sp. CCGE-LA001]
ATGCCCCGCACACCGATTCATCCCGGCGAACAGCTTGCCGAAGAACTGCGCGAACTCGGCATCACGGCCGCGGAACTGGC
GCGCCAGATCGATGTGCCGGTCAATCGCGTCACCGGCATCATCAACGGGCAGCGCGGCATCACCGCTGACACGGCGCTGC
GTCTAGGTCATTGGTTCGGTACCAGCCCGCAATTCTGGATGAACCTGCAACAGCAATATGAGCTGCGGTTGGCGGAGAAG
GAGATTGGCGCGCAGGTTGCGTCGCTGCCCCGCCGCACAAATGCGCAGTCGACACGAAAGCTTGGAAAAACCGCATGA

Structures

Toxin

• AlphaFold DB: A0A0X8CJV5