Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 139491..140027 Replicon plasmid pPsCRG01-1
Accession NZ_CP013946
Organism Piscirickettsia salmonis strain PSCGR01

Toxin (Protein)


Gene name yoeB Uniprot ID A0A1L6THY5
Locus tag AWE47_RS16855 Protein ID WP_036772441.1
Coordinates 139755..140027 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A0B8U9U2
Locus tag AWE47_RS16850 Protein ID WP_032126795.1
Coordinates 139491..139751 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWE47_RS16820 135307..135996 + 690 WP_017375909.1 hypothetical protein -
AWE47_RS16825 136044..136859 + 816 WP_036775032.1 hypothetical protein -
AWE47_RS19205 136943..137149 + 207 Protein_175 IS6 family transposase -
AWE47_RS20490 137200..137367 - 168 Protein_176 IS6 family transposase -
AWE47_RS16835 137437..138069 + 633 Protein_177 IS6-like element ISPsa2 family transposase -
AWE47_RS16840 138135..138701 - 567 Protein_178 IS6 family transposase -
AWE47_RS20495 138987..139151 - 165 Protein_179 IS6 family transposase -
AWE47_RS16850 139491..139751 + 261 WP_032126795.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AWE47_RS16855 139755..140027 + 273 WP_036772441.1 Txe/YoeB family addiction module toxin Toxin
AWE47_RS16860 140103..140831 + 729 WP_017375910.1 IS6 family transposase -
AWE47_RS16865 141400..142464 - 1065 WP_174872554.1 IS91 family transposase -
AWE47_RS21205 142644..142823 - 180 WP_157894752.1 hypothetical protein -
AWE47_RS16875 143236..144063 + 828 WP_048876208.1 IS30 family transposase -
AWE47_RS16880 144106..144835 - 730 Protein_186 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..179831 179831
- inside IScluster/Tn - - 133444..150449 17005


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(7-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-74)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10785.31 Da        Isoelectric Point: 7.8926

>T59638 WP_036772441.1 NZ_CP013946:139755-140027 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRMPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
ISACRYHYDQ

Download         Length: 273 bp

>T59638 NZ_CP013946:139755-140027 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
GCTTAAAAAAATCAATAAACTTATAAAGGAATGTCAACGCATGCCGACAGAAGGTATTGGTCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGCTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTTCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9555.93 Da        Isoelectric Point: 5.1167

>AT59638 WP_032126795.1 NZ_CP013946:139491-139751 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Download         Length: 261 bp

>AT59638 NZ_CP013946:139491-139751 [Piscirickettsia salmonis]
ATGCTACGAGATAACATCAATGCTACGGCAGCACGGGATAATTTTAAGAGTACCTGTGATACAGTGTGTGAAAACAATGC
TCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGTATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L6THY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B8U9U2

References