Detailed information of TA system TA059628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4256078..4256688
• Replicon: chromosome
• Accession: NZ_CP013940
• Organism: Cronobacter malonaticus LMG 23826

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V5TV61
• Locus tag: AFK66_RS19880
• Protein ID: WP_007779945.1
• Coordinates: 4256078..4256398 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AFK66_RS19885
• Protein ID: WP_007779948.1
• Coordinates: 4256398..4256688 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK66_RS19865	4253074..4254225	+	1152	WP_032983190.1	L-threonine dehydrogenase	-
AFK66_RS19870	4254250..4255122	-	873	WP_023897763.1	ROK family protein	-
AFK66_RS19875	4255279..4255878	+	600	WP_007779941.1	LysE family transporter	-
AFK66_RS19880	4256078..4256398	+	321	WP_007779945.1	hypothetical protein	Toxin
AFK66_RS19885	4256398..4256688	+	291	WP_007779948.1	helix-turn-helix domain-containing protein	Antitoxin
AFK66_RS19890	4256762..4257691	-	930	WP_023897767.1	formate dehydrogenase accessory protein FdhE	-
AFK66_RS19895	4257688..4258323	-	636	WP_004385165.1	formate dehydrogenase cytochrome b556 subunit	-
AFK66_RS19900	4258320..4259222	-	903	WP_004385164.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12396.41 Da
• Isoelectric Point: 10.2290

>T59628 WP_007779945.1 NZ_CP013940:4256078-4256398 [Cronobacter malonaticus LMG 23826]
MLFIETEIFTRAVAALLSDDDYQRLQRLQWFLALRPDYGDVIPDTGGLRKARWLAGGRGKRGGVRIIYFYRAAADEIRLL
LIYRKGIKDDLSPHEKSVLRMLNERW

Nucleotide

• Length: 321 bp

>T59628 NZ_CP013940:4256078-4256398 [Cronobacter malonaticus LMG 23826]
ATGCTCTTCATTGAAACGGAAATCTTTACCCGGGCCGTGGCCGCCCTGCTCAGTGATGACGATTACCAGCGGTTGCAGCG
GTTGCAGTGGTTCCTGGCCCTGCGGCCCGATTACGGTGATGTTATCCCGGATACCGGCGGGCTTCGTAAAGCTCGCTGGC
TTGCAGGCGGACGGGGCAAACGAGGCGGCGTCAGGATCATCTATTTTTATCGCGCTGCTGCCGATGAAATCCGCCTGCTG
CTGATTTATCGCAAGGGGATAAAAGACGATCTCTCGCCTCACGAAAAATCTGTGCTCAGAATGCTGAATGAGAGGTGGTG
A

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10674.16 Da
• Isoelectric Point: 10.4419

>AT59628 WP_007779948.1 NZ_CP013940:4256398-4256688 [Cronobacter malonaticus LMG 23826]
MDNALFEQLTESLRQMNDISEGRREPSRAFVIDALKIKEIRQASGLSQTKFASLISVSVDTLRNWEQGRRSPTGPARALL
RAIANDPQHVISALAL

Nucleotide

• Length: 291 bp

>AT59628 NZ_CP013940:4256398-4256688 [Cronobacter malonaticus LMG 23826]
ATGGACAACGCGCTGTTTGAACAGTTAACCGAAAGCCTGCGGCAGATGAATGATATCAGCGAAGGCCGCCGCGAGCCTTC
ACGGGCTTTTGTCATCGACGCGCTGAAAATAAAAGAGATCAGACAGGCATCAGGGCTTTCTCAGACGAAATTTGCCAGCC
TGATTTCGGTAAGTGTGGATACGCTGCGCAACTGGGAGCAGGGAAGGCGTTCGCCGACGGGGCCCGCGCGTGCGCTGTTG
CGTGCGATTGCGAACGATCCGCAGCATGTTATCTCTGCACTGGCGTTGTAA

Structures

Toxin

• AlphaFold DB: V5TV61