Detailed information of TA system TA059625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2088769..2089334
• Replicon: chromosome
• Accession: NZ_CP013940
• Organism: Cronobacter malonaticus LMG 23826

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V5U0Z2
• Locus tag: AFK66_RS09930
• Protein ID: WP_023898792.1
• Coordinates: 2089056..2089334 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: AFK66_RS09925
• Protein ID: WP_032968184.1
• Coordinates: 2088769..2089056 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFK66_RS09910	2084361..2085227	+	867	WP_007774909.1	ABC transporter permease	-
AFK66_RS09915	2085256..2086314	-	1059	WP_007774908.1	YncE family protein	-
AFK66_RS09920	2086592..2088709	+	2118	WP_023898790.1	TonB-dependent receptor	-
AFK66_RS09925	2088769..2089056	-	288	WP_032968184.1	HigA family addiction module antidote protein	Antitoxin
AFK66_RS09930	2089056..2089334	-	279	WP_023898792.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AFK66_RS09935	2089633..2091237	+	1605	WP_007774898.1	alpha-amylase family protein	-
AFK66_RS09940	2091239..2092225	-	987	WP_007774884.1	SDR family oxidoreductase	-
AFK66_RS09945	2092337..2094271	-	1935	WP_007774882.1	HAMP domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10580.05 Da
• Isoelectric Point: 10.2970

>T59625 WP_023898792.1 NZ_CP013940:c2089334-2089056 [Cronobacter malonaticus LMG 23826]
MIRSFRHKGLQRYFETGSTAGIHARHARKISLRLAVLNQAIRPADVDLPGFFLHPLTGDRKGIWAVTVSGNWRITFEFRD
GDVFIVNDEDYH

Nucleotide

• Length: 279 bp

>T59625 NZ_CP013940:c2089334-2089056 [Cronobacter malonaticus LMG 23826]
ATGATCAGAAGCTTCAGGCATAAAGGCCTGCAACGCTATTTCGAAACGGGTTCCACCGCAGGCATTCATGCCCGCCATGC
CAGAAAGATTTCTCTGCGGCTGGCGGTGCTGAATCAGGCGATAAGACCCGCTGACGTCGACTTACCGGGCTTTTTCCTGC
ATCCCCTGACGGGGGATCGTAAAGGTATATGGGCTGTGACCGTCAGCGGAAACTGGCGCATCACGTTTGAGTTTCGGGAT
GGCGATGTCTTCATCGTCAACGATGAGGATTACCACTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10437.04 Da
• Isoelectric Point: 8.7963

>AT59625 WP_032968184.1 NZ_CP013940:c2089056-2088769 [Cronobacter malonaticus LMG 23826]
MATMFNPPHPGRLVQESMEALGLSARALAKALDVAPSTVQRLLVGKSDVSPEMALRLSAVLGSSPHVWLGMQNEYDLFHA
RQSVDFSHLQRLQRA

Nucleotide

• Length: 288 bp

>AT59625 NZ_CP013940:c2089056-2088769 [Cronobacter malonaticus LMG 23826]
ATGGCAACGATGTTTAACCCACCACACCCAGGCAGGCTGGTGCAGGAATCAATGGAAGCGCTCGGCCTCAGCGCCCGCGC
GCTGGCCAAAGCGCTGGACGTCGCGCCGTCAACAGTGCAGCGGCTGCTGGTCGGCAAATCTGACGTCTCCCCGGAAATGG
CGCTGCGTCTCTCGGCAGTCCTTGGCAGCTCGCCGCATGTCTGGCTGGGTATGCAGAATGAATACGATCTCTTTCATGCG
CGCCAGTCTGTTGATTTCTCGCATCTGCAACGGTTGCAGAGGGCATAA

Structures

Toxin

• AlphaFold DB: V5U0Z2