Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1295881..1296528 Replicon chromosome
Accession NZ_CP013940
Organism Cronobacter malonaticus LMG 23826

Toxin (Protein)


Gene name higB Uniprot ID V5TX81
Locus tag AFK66_RS06055 Protein ID WP_007776552.1
Coordinates 1295881..1296228 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V5TYW5
Locus tag AFK66_RS06060 Protein ID WP_007776551.1
Coordinates 1296229..1296528 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFK66_RS06030 1291457..1292467 + 1011 WP_004387466.1 galactose/methyl galactoside ABC transporter permease MglC -
AFK66_RS06035 1292683..1293395 - 713 Protein_1199 outer membrane permeability protein SanA -
AFK66_RS06040 1293520..1294404 - 885 WP_007776555.1 cytidine deaminase -
AFK66_RS06045 1294534..1295229 - 696 WP_007776554.1 CidB/LrgB family autolysis modulator -
AFK66_RS06050 1295226..1295627 - 402 WP_007776553.1 CidA/LrgA family protein -
AFK66_RS06055 1295881..1296228 + 348 WP_007776552.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AFK66_RS06060 1296229..1296528 + 300 WP_007776551.1 XRE family transcriptional regulator Antitoxin
AFK66_RS06065 1296599..1297531 + 933 WP_007776525.1 tRNA dihydrouridine(16) synthase DusC -
AFK66_RS06070 1297534..1298457 - 924 WP_007776522.1 LysR family transcriptional regulator -
AFK66_RS06075 1298533..1299489 + 957 WP_032983658.1 MBL fold metallo-hydrolase -
AFK66_RS21200 1299526..1299609 - 84 WP_000691708.1 protein YohP -
AFK66_RS06080 1299782..1300555 + 774 WP_007794933.1 SDR family oxidoreductase -
AFK66_RS06085 1300552..1301118 - 567 WP_032968930.1 DedA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1285539..1334605 49066


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-83)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13339.54 Da        Isoelectric Point: 9.8707

>T59615 WP_007776552.1 NZ_CP013940:1295881-1296228 [Cronobacter malonaticus LMG 23826]
MWTIYLTHEFECWLARQPQPLQEDVLAALGLLKIKGPHLGHPYADTLKGSRHAGMKELRVQHAGGPIRAFYAFDPHRYAI
VLCAAEKKGDEKRFYRVMLRVANKLFSHYLNHREV

Download         Length: 348 bp

>T59615 NZ_CP013940:1295881-1296228 [Cronobacter malonaticus LMG 23826]
ATGTGGACTATTTACCTCACACACGAGTTTGAGTGCTGGCTCGCGAGGCAGCCGCAGCCGTTGCAGGAAGATGTGCTGGC
GGCGCTGGGGCTTTTGAAAATCAAAGGCCCGCACCTCGGGCATCCGTATGCCGATACGCTCAAAGGCTCGCGTCACGCAG
GCATGAAGGAATTACGGGTTCAGCACGCCGGGGGGCCCATACGTGCCTTTTACGCTTTTGATCCGCACCGTTACGCCATT
GTGCTGTGCGCGGCAGAGAAAAAGGGCGATGAAAAGCGCTTTTACCGGGTCATGCTGCGCGTCGCTAACAAACTGTTTAG
CCATTATCTGAACCACCGGGAGGTATAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11035.67 Da        Isoelectric Point: 5.1667

>AT59615 WP_007776551.1 NZ_CP013940:1296229-1296528 [Cronobacter malonaticus LMG 23826]
MKTFDEVLAAQTPEARARIEEKTAQLLLETHLYALREALSVSQTEMARQMGIAQPSVAAIEQRGNEMKISTLKRYVEALG
GTVRLDVELPDGQRFGFTL

Download         Length: 300 bp

>AT59615 NZ_CP013940:1296229-1296528 [Cronobacter malonaticus LMG 23826]
ATGAAGACGTTTGATGAGGTGCTGGCGGCGCAGACGCCGGAGGCCCGCGCGCGTATTGAAGAGAAAACGGCGCAGCTGCT
GCTGGAAACCCACCTTTATGCGCTGCGCGAGGCGCTCAGCGTGTCGCAAACCGAGATGGCGCGCCAGATGGGTATCGCAC
AACCGTCGGTCGCGGCTATTGAACAGCGCGGCAACGAGATGAAAATCTCGACGCTGAAGCGCTATGTCGAAGCTCTGGGC
GGCACGGTGCGGCTGGATGTCGAGCTGCCGGACGGCCAGCGTTTCGGTTTTACGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V5TX81

Antitoxin

Source ID Structure
AlphaFold DB A0A2S9UA72

References