Detailed information of TA system TA059576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-DinJ
• Location: 1162703..1163267
• Replicon: chromosome
• Accession: NZ_CP013921
• Organism: Lacticaseibacillus paracasei strain KL1

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: C2FG57
• Locus tag: AWC33_RS05595
• Protein ID: WP_003661092.1
• Coordinates: 1162703..1163005 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A8E0I752
• Locus tag: AWC33_RS05600
• Protein ID: WP_016365698.1
• Coordinates: 1162998..1163267 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWC33_RS05580	1158386..1158823	+	438	WP_003661085.1	hypothetical protein	-
AWC33_RS05585	1158998..1161175	-	2178	WP_016383421.1	RNA-binding transcriptional accessory protein	-
AWC33_RS05590	1161411..1162103	-	693	WP_003661089.1	hypothetical protein	-
AWC33_RS05595	1162703..1163005	-	303	WP_003661092.1	type II toxin-antitoxin system YafQ family toxin	Toxin
AWC33_RS05600	1162998..1163267	-	270	WP_016365698.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AWC33_RS05605	1163641..1164294	-	654	WP_003570049.1	GNAT family N-acetyltransferase	-
AWC33_RS05610	1164339..1165154	-	816	WP_003661098.1	DUF2399 domain-containing protein	-
AWC33_RS14775	1165179..1165802	-	624	WP_079351422.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-97)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (10-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11383.23 Da
• Isoelectric Point: 10.4225

>T59576 WP_003661092.1 NZ_CP013921:c1163005-1162703 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGGWLLIYKIK
QQDLILTLVRTGSHHNLLGK

Nucleotide

• Length: 303 bp

>T59576 NZ_CP013921:c1163005-1162703 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCTGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCTT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTACATGTTGACGGCCCTCGTGGCGGCTGGTTGCTAATCTATAAAATTAAG
CAGCAAGATCTTATTTTAACCCTAGTTAGAACCGGATCTCATCATAACCTTCTGGGTAAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9996.71 Da
• Isoelectric Point: 7.2026

>AT59576 WP_016365698.1 NZ_CP013921:c1163267-1162998 [Lacticaseibacillus paracasei]
MAATKKETRLNIRIDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Nucleotide

• Length: 270 bp

>AT59576 NZ_CP013921:c1163267-1162998 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAAGAAACTCGCTTGAATATTCGTATTGATCCTGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACGATGTTCATGACGAAAATGGTGAAGGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACACCT
GATGACATGTGGAGAGACTTGAATGTATAG