Detailed information of TA system TA059471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1433995..1434580
• Replicon: chromosome
• Accession: NZ_CP013854
• Organism: Pseudonocardia sp. HH130630-07

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A1B1ZVQ5
• Locus tag: AFB00_RS06835
• Protein ID: WP_068796532.1
• Coordinates: 1433995..1434279 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AFB00_RS06840
• Protein ID: WP_068800070.1
• Coordinates: 1434299..1434580 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFB00_RS06795	1429393..1430004	+	612	WP_068800068.1	FMN-binding negative transcriptional regulator	-
AFB00_RS06800	1430013..1430444	-	432	WP_083275982.1	GNAT family N-acetyltransferase	-
AFB00_RS31490	1430866..1431763	-	898	Protein_1350	IS5 family transposase	-
AFB00_RS06815	1431810..1432181	-	372	WP_197519892.1	pyridoxamine 5'-phosphate oxidase family protein	-
AFB00_RS06820	1432230..1432859	-	630	WP_068796529.1	dihydrofolate reductase family protein	-
AFB00_RS06825	1433058..1433498	+	441	WP_068796530.1	cupin domain-containing protein	-
AFB00_RS06830	1433486..1433902	+	417	WP_197519773.1	antibiotic biosynthesis monooxygenase	-
AFB00_RS06835	1433995..1434279	+	285	WP_068796532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AFB00_RS06840	1434299..1434580	+	282	WP_068800070.1	HigA family addiction module antidote protein	Antitoxin
AFB00_RS06845	1434728..1435312	+	585	WP_068796533.1	TMEM165/GDT1 family protein	-
AFB00_RS06855	1435499..1436317	+	819	WP_083275321.1	class I SAM-dependent methyltransferase	-
AFB00_RS06860	1436304..1437035	-	732	WP_068796535.1	uracil-DNA glycosylase	-
AFB00_RS06865	1437032..1437991	-	960	WP_197519774.1	Ppx/GppA family phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-83)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-61)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10789.16 Da
• Isoelectric Point: 8.0884

>T59471 WP_068796532.1 NZ_CP013854:1433995-1434279 [Pseudonocardia sp. HH130630-07]
VLRSFADKATEQIWRREHVRRLHPDVARTALRKLAILDAAATLDDADSPPGNRLELLKGDRAGQHSIRVNEQYRICFRWT
AGGPEDVELTKHYE

Nucleotide

• Length: 285 bp

>T59471 NZ_CP013854:1433995-1434279 [Pseudonocardia sp. HH130630-07]
GTGTTGCGGAGTTTCGCGGACAAGGCGACCGAGCAGATCTGGCGGCGCGAGCACGTACGCCGGTTGCACCCGGACGTCGC
CCGTACGGCACTCCGCAAGCTGGCGATTCTCGACGCGGCTGCCACACTCGACGATGCGGACAGCCCGCCCGGGAACCGCC
TCGAACTCCTGAAGGGCGATCGCGCGGGGCAGCACAGCATCCGGGTCAACGAGCAGTACCGGATCTGCTTTCGCTGGACT
GCGGGTGGCCCGGAGGACGTCGAGCTCACGAAGCACTACGAGTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10515.09 Da
• Isoelectric Point: 4.7815

>AT59471 WP_068800070.1 NZ_CP013854:1434299-1434580 [Pseudonocardia sp. HH130630-07]
MEPISPGEILVEEYMRPYGLSQNAVAAAIGVDPMRISQIVRGTRAISADTALRLSRLFGTSDLFWLNLQDRYEIERLHDT
MGPVLERIEPVRT

Nucleotide

• Length: 282 bp

>AT59471 NZ_CP013854:1434299-1434580 [Pseudonocardia sp. HH130630-07]
ATGGAGCCGATCTCGCCCGGCGAGATCCTGGTCGAGGAGTACATGAGGCCGTACGGGCTCTCCCAGAACGCCGTGGCGGC
CGCGATCGGCGTCGACCCCATGCGGATCAGCCAGATCGTCCGTGGCACGCGCGCGATCAGCGCCGACACGGCACTCCGGT
TGTCGCGCCTGTTCGGCACGTCCGACCTGTTCTGGTTGAACCTCCAGGATCGCTACGAGATCGAGCGGCTGCACGACACC
ATGGGCCCGGTCCTGGAAAGGATCGAGCCGGTACGCACCTGA

Structures

Toxin

• AlphaFold DB: A0A1B1ZVQ5