Detailed information of TA system TA059433

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40452..40705
• Replicon: plasmid pJJ2434_2
• Accession: NZ_CP013834
• Organism: Escherichia coli strain JJ2434

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: AVR68_RS26800
• Protein ID: WP_001312851.1
• Coordinates: 40452..40601 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	40646..40705 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVR68_RS00990	36427..37093	+	667	Protein_55	hypothetical protein	-
AVR68_RS00995	37129..37428	-	300	WP_001528606.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AVR68_RS01000	37418..37681	-	264	WP_001089473.1	hypothetical protein	-
AVR68_RS28950	37788..38048	-	261	WP_019842130.1	hypothetical protein	-
AVR68_RS01010	38750..39607	-	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-
AVR68_RS27020	39600..39674	-	75	WP_001365705.1	RepA leader peptide Tap	-
AVR68_RS01020	39919..40167	-	249	WP_000083839.1	replication regulatory protein RepA	-
AVR68_RS26800	40452..40601	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	40646..40705	+	60	NuclAT_1	-	Antitoxin
-	40646..40705	+	60	NuclAT_1	-	Antitoxin
-	40646..40705	+	60	NuclAT_1	-	Antitoxin
-	40646..40705	+	60	NuclAT_1	-	Antitoxin
AVR68_RS01025	40848..41321	-	474	WP_016240489.1	hypothetical protein	-
AVR68_RS01030	41475..42065	-	591	WP_033807967.1	DUF2726 domain-containing protein	-
AVR68_RS27040	42103..42312	-	210	WP_071888758.1	hemolysin expression modulator Hha	-
AVR68_RS01035	42381..42818	-	438	Protein_66	thermonuclease family protein	-
AVR68_RS01040	43063..43275	-	213	WP_052983100.1	ANR family transcriptional regulator	-
AVR68_RS01045	43408..43969	-	562	Protein_68	fertility inhibition protein FinO	-
AVR68_RS01050	44024..44770	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..62183	62183

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T59433 WP_001312851.1 NZ_CP013834:c40601-40452 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T59433 NZ_CP013834:c40601-40452 [Escherichia coli]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT59433 NZ_CP013834:40646-40705 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin