Detailed information of TA system TA059430

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 11231..11657
• Replicon: plasmid pJJ2434_2
• Accession: NZ_CP013834
• Organism: Escherichia coli strain JJ2434

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AVR68_RS26970
• Protein ID: WP_001312861.1
• Coordinates: 11231..11389 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	11433..11657 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVR68_RS00775	6307..6546	-	240	WP_059219409.1	conjugal transfer relaxosome protein TraY	-
AVR68_RS00780	6655..7302	-	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
AVR68_RS00785	7493..7877	-	385	Protein_15	relaxosome protein TraM	-
AVR68_RS00790	8210..8800	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
AVR68_RS00795	9096..9916	-	821	Protein_17	DUF945 domain-containing protein	-
AVR68_RS00800	10030..10320	-	291	WP_149010534.1	hypothetical protein	-
AVR68_RS00810	10619..10993	+	375	Protein_19	hypothetical protein	-
AVR68_RS26970	11231..11389	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	11433..11657	-	225	NuclAT_0	-	Antitoxin
-	11433..11657	-	225	NuclAT_0	-	Antitoxin
-	11433..11657	-	225	NuclAT_0	-	Antitoxin
-	11433..11657	-	225	NuclAT_0	-	Antitoxin
AVR68_RS26975	11469..11648	+	180	WP_001309233.1	hypothetical protein	-
AVR68_RS29615	11626..11782	-	157	Protein_22	plasmid SOS inhibition protein A	-
AVR68_RS00815	11863..12513	+	651	WP_000993921.1	IS66 family insertion sequence hypothetical protein	-
AVR68_RS00820	12513..12860	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
AVR68_RS00825	12880..14451	+	1572	WP_059219400.1	IS66 family transposase	-
AVR68_RS00830	14483..15099	-	617	Protein_26	plasmid SOS inhibition protein A	-
AVR68_RS00835	15096..15530	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..62183	62183

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T59430 WP_001312861.1 NZ_CP013834:c11389-11231 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T59430 NZ_CP013834:c11389-11231 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT59430 NZ_CP013834:c11657-11433 [Escherichia coli]
TCGCACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin