Detailed information of TA system TA059424

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 24534..24803
• Replicon: plasmid pJJ2434_1
• Accession: NZ_CP013833
• Organism: Escherichia coli strain JJ2434

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AVR68_RS00145
• Protein ID: WP_001312861.1
• Coordinates: 24645..24803 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	24534..24599 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVR68_RS30180	20183..20301	+	119	Protein_23	single-stranded DNA-binding protein	-
AVR68_RS00115	20327..20854	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
AVR68_RS00120	20910..21143	+	234	WP_000006004.1	DUF905 domain-containing protein	-
AVR68_RS00125	21202..23160	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
AVR68_RS00130	23215..23649	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
AVR68_RS00135	23646..24365	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
AVR68_RS30185	24377..24565	-	189	WP_001299721.1	hypothetical protein	-
-	24377..24601	+	225	NuclAT_0	-	-
-	24377..24601	+	225	NuclAT_0	-	-
-	24377..24601	+	225	NuclAT_0	-	-
-	24377..24601	+	225	NuclAT_0	-	-
-	24534..24599	+	66	-	-	Antitoxin
AVR68_RS00145	24645..24803	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
AVR68_RS26885	25251..25430	-	180	Protein_31	hypothetical protein	-
AVR68_RS00150	25481..26227	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
AVR68_RS00155	26242..27783	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
AVR68_RS00160	28308..28595	+	288	WP_000107537.1	hypothetical protein	-
AVR68_RS00165	28716..29537	+	822	WP_001234469.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / tet(A)	-	1..126302	126302

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T59424 WP_001312861.1 NZ_CP013833:24645-24803 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T59424 NZ_CP013833:24645-24803 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT59424 NZ_CP013833:24534-24599 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin