Detailed information of TA system TA059417

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 120534..120960
• Replicon: plasmid pCD306
• Accession: NZ_CP013832
• Organism: Escherichia coli strain CD306

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AVR67_RS26140
• Protein ID: WP_001312861.1
• Coordinates: 120534..120692 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	120736..120960 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVR67_RS26095	115572..115799	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
AVR67_RS26100	115894..116580	-	687	WP_000332487.1	PAS domain-containing protein	-
AVR67_RS26105	116774..117157	-	384	WP_001151564.1	relaxosome protein TraM	-
AVR67_RS26110	117443..118090	+	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
AVR67_RS26115	118387..119208	-	822	WP_001234469.1	DUF945 domain-containing protein	-
AVR67_RS26120	119328..119615	-	288	WP_000107537.1	hypothetical protein	-
AVR67_RS26140	120534..120692	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	120736..120960	-	225	NuclAT_0	-	Antitoxin
-	120736..120960	-	225	NuclAT_0	-	Antitoxin
-	120736..120960	-	225	NuclAT_0	-	Antitoxin
-	120736..120960	-	225	NuclAT_0	-	Antitoxin
AVR67_RS29580	120772..120960	+	189	WP_001299721.1	hypothetical protein	-
AVR67_RS26150	120972..121691	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
AVR67_RS26155	121688..122122	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
AVR67_RS26160	122177..124135	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
AVR67_RS26165	124194..124427	-	234	WP_000006004.1	DUF905 domain-containing protein	-
AVR67_RS26170	124483..125010	-	528	WP_000290793.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / aadA5 / sitABCD / blaTEM-1B	iucA / iucB / iucB / iucC / iucD / iutA	1..145221	145221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T59417 WP_001312861.1 NZ_CP013832:c120692-120534 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T59417 NZ_CP013832:c120692-120534 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT59417 NZ_CP013832:c120960-120736 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin