Detailed information of TA system TA059388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 574584..575263
• Replicon: chromosome
• Accession: NZ_CP013831
• Organism: Escherichia coli strain CD306

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1PK60
• Locus tag: AVR67_RS02790
• Protein ID: WP_000057523.1
• Coordinates: 574584..574886 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: AVR67_RS02795
• Protein ID: WP_000806442.1
• Coordinates: 574922..575263 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVR67_RS02735	569655..569924	+	270	WP_000843446.1	hypothetical protein	-
AVR67_RS02740	569952..570482	+	531	WP_001381528.1	host-nuclease inhibitor Gam family protein	-
AVR67_RS02750	570771..571043	+	273	WP_042203510.1	hypothetical protein	-
AVR67_RS02755	571053..571358	+	306	WP_000197789.1	hypothetical protein	-
AVR67_RS02760	571355..572038	+	684	WP_000131939.1	DUF2786 domain-containing protein	-
AVR67_RS02765	572035..572265	+	231	WP_000631814.1	hypothetical protein	-
AVR67_RS02770	572255..572470	+	216	WP_000123379.1	hypothetical protein	-
AVR67_RS02775	572463..572912	+	450	WP_001573929.1	regulatory protein GemA	-
AVR67_RS02780	572884..573282	+	399	WP_001281696.1	DNA-binding protein	-
AVR67_RS02785	573397..574029	+	633	WP_001571573.1	hypothetical protein	-
AVR67_RS29430	574033..574194	-	162	WP_001571575.1	hypothetical protein	-
AVR67_RS02790	574584..574886	+	303	WP_000057523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AVR67_RS02795	574922..575263	+	342	WP_000806442.1	HigA family addiction module antidote protein	Antitoxin
AVR67_RS02800	575321..577825	-	2505	WP_000083947.1	copper-exporting P-type ATPase CopA	-
AVR67_RS02805	578087..579019	+	933	WP_000883041.1	glutaminase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	535827..575263	39436

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11809.44 Da
• Isoelectric Point: 10.3980

>T59388 WP_000057523.1 NZ_CP013831:574584-574886 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T59388 NZ_CP013831:574584-574886 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT59388 WP_000806442.1 NZ_CP013831:574922-575263 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT59388 NZ_CP013831:574922-575263 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: S1PK60

Antitoxin

• AlphaFold DB: S1QAY3