Detailed information of TA system TA059357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 142309..142845
• Replicon: plasmid p3PS13
• Accession: NZ_CP013804
• Organism: Piscirickettsia salmonis strain PM22180B

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A1L6THY5
• Locus tag: AVM70_RS17720
• Protein ID: WP_036772441.1
• Coordinates: 142573..142845 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A0B8U9U2
• Locus tag: AVM70_RS17715
• Protein ID: WP_032126795.1
• Coordinates: 142309..142569 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVM70_RS17680	138125..138814	+	690	WP_017375909.1	hypothetical protein	-
AVM70_RS17685	138862..139677	+	816	WP_075275477.1	hypothetical protein	-
AVM70_RS17690	139761..139967	+	207	Protein_181	IS6 family transposase	-
AVM70_RS19760	140018..140185	-	168	Protein_182	IS6 family transposase	-
AVM70_RS17700	140255..140887	+	633	Protein_183	IS6-like element ISPsa2 family transposase	-
AVM70_RS17705	140953..141519	-	567	Protein_184	IS6 family transposase	-
AVM70_RS20525	141687..141836	-	150	WP_196771747.1	hypothetical protein	-
AVM70_RS19765	141805..141969	-	165	Protein_186	IS6 family transposase	-
AVM70_RS17715	142309..142569	+	261	WP_032126795.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AVM70_RS17720	142573..142845	+	273	WP_036772441.1	Txe/YoeB family addiction module toxin	Toxin
AVM70_RS17725	142921..143649	+	729	WP_017375910.1	IS6 family transposase	-
AVM70_RS17730	144219..145283	-	1065	WP_174872554.1	IS91 family transposase	-
AVM70_RS20530	145463..145642	-	180	WP_157894752.1	hypothetical protein	-
AVM70_RS20535	145775..145975	-	201	WP_196426542.1	transposase zinc-binding domain-containing protein	-
AVM70_RS17745	146055..146882	+	828	WP_048876208.1	IS30 family transposase	-
AVM70_RS17750	146925..147654	-	730	Protein_194	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..181038	181038
	-	inside	IScluster/Tn	-	-	136262..153268	17006

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (7-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-74)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10785.31 Da
• Isoelectric Point: 7.8926

>T59357 WP_036772441.1 NZ_CP013804:142573-142845 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRMPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
ISACRYHYDQ

Nucleotide

• Length: 273 bp

>T59357 NZ_CP013804:142573-142845 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
GCTTAAAAAAATCAATAAACTTATAAAGGAATGTCAACGCATGCCGACAGAAGGTATTGGTCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGCTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTTCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9555.93 Da
• Isoelectric Point: 5.1167

>AT59357 WP_032126795.1 NZ_CP013804:142309-142569 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Nucleotide

• Length: 261 bp

>AT59357 NZ_CP013804:142309-142569 [Piscirickettsia salmonis]
ATGCTACGAGATAACATCAATGCTACGGCAGCACGGGATAATTTTAAGAGTACCTGTGATACAGTGTGTGAAAACAATGC
TCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGTATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A1L6THY5

Antitoxin

• AlphaFold DB: A0A0B8U9U2