Detailed information of TA system TA059226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 48370..49097
• Replicon: plasmid 2
• Accession: NZ_CP013713
• Organism: Klebsiella pneumoniae strain J1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: AU361_RS26840
• Protein ID: WP_011251285.1
• Coordinates: 48370..48681 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AU361_RS26845
• Protein ID: WP_011251286.1
• Coordinates: 48678..49097 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AU361_RS26805	43465..43725	-	261	WP_017901409.1	hypothetical protein	-
AU361_RS28410	44297..44674	-	378	Protein_51	transposase	-
AU361_RS26815	44808..45776	+	969	WP_077265492.1	IS5 family transposase	-
AU361_RS26820	45834..46883	-	1050	Protein_53	IS66 family transposase	-
AU361_RS26825	46932..47279	-	348	WP_004114612.1	IS66 family insertion sequence element accessory protein TnpB	-
AU361_RS26830	47276..47614	-	339	WP_023288266.1	IS66 family insertion sequence hypothetical protein	-
AU361_RS26835	47707..48165	+	459	Protein_56	DDE-type integrase/transposase/recombinase	-
AU361_RS26840	48370..48681	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
AU361_RS26845	48678..49097	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
AU361_RS28420	49244..50210	+	967	Protein_59	IS5 family transposase	-
AU361_RS26860	50354..51040	-	687	Protein_60	hypothetical protein	-
AU361_RS26865	51040..51504	-	465	WP_032435691.1	hypothetical protein	-
AU361_RS29940	52098..52853	+	756	Protein_62	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..53400	53400
	-	inside	IScluster/Tn	-	-	44853..50210	5357

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T59226 WP_011251285.1 NZ_CP013713:48370-48681 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T59226 NZ_CP013713:48370-48681 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT59226 WP_011251286.1 NZ_CP013713:48678-49097 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT59226 NZ_CP013713:48678-49097 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1