Detailed information of TA system TA059202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 465860..466455
• Replicon: chromosome
• Accession: NZ_CP013696
• Organism: Pseudomonas aeruginosa strain 12-4-4(59)

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: AU380_RS29595
• Protein ID: WP_003113526.1
• Coordinates: 466177..466455 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AU380_RS02145
• Protein ID: WP_003113527.1
• Coordinates: 465860..466165 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AU380_RS02110	461000..461848	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
AU380_RS02120	462015..462956	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
AU380_RS02125	463073..463687	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
AU380_RS02130	463729..464313	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
AU380_RS02135	464354..465454	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
AU380_RS02145	465860..466165	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
AU380_RS29595	466177..466455	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AU380_RS02150	466784..469012	+	2229	WP_058150624.1	TonB-dependent receptor	-
AU380_RS02155	469082..469729	-	648	WP_003095021.1	carbonate dehydratase	-
AU380_RS02160	469791..471029	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T59202 WP_003113526.1 NZ_CP013696:c466455-466177 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T59202 NZ_CP013696:c466455-466177 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTAG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT59202 WP_003113527.1 NZ_CP013696:c466165-465860 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT59202 NZ_CP013696:c466165-465860 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3