Detailed information of TA system TA059170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 849775..850430
• Replicon: chromosome
• Accession: NZ_CP013667
• Organism: Coxiella burnetii strain 3262

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q83BL3
• Locus tag: AUR58_RS04805
• Protein ID: WP_010958264.1
• Coordinates: 849775..850113 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AUR58_RS04810
• Protein ID: WP_010958263.1
• Coordinates: 850119..850430 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AUR58_RS04785	844847..845866	-	1020	WP_005772222.1	IS110-like element IS1111A family transposase	-
AUR58_RS12345	845865..846104	+	240	WP_012220681.1	hypothetical protein	-
AUR58_RS04795	846270..847943	-	1674	WP_012220680.1	CBU_1493 family Dot/Icm T4SS effector	-
AUR58_RS04800	848271..849416	-	1146	WP_040953472.1	ISAs1 family transposase	-
AUR58_RS04805	849775..850113	+	339	WP_010958264.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AUR58_RS04810	850119..850430	+	312	WP_010958263.1	HigA family addiction module antidote protein	Antitoxin
AUR58_RS04815	850499..851230	-	732	WP_010958262.1	UDP-2,3-diacylglucosamine diphosphatase	-
AUR58_RS04820	851387..852784	+	1398	WP_010958261.1	glutamate--tRNA ligase	-
AUR58_RS04825	852775..854157	+	1383	WP_010958260.1	cysteine--tRNA ligase	-
AUR58_RS04830	854253..855272	+	1020	WP_005772222.1	IS110-like element IS1111A family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	CBU_1493	844847..867765	22918

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (26-72)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13409.66 Da
• Isoelectric Point: 10.4588

>T59170 WP_010958264.1 NZ_CP013667:849775-850113 [Coxiella burnetii]
MPLTLYIMLDVTRKTVILEVIIKSFKDKYTKYLYKGVSVSKWQAIRKQAERRLQILDSVTSLDDLRSLPSNRFESLRGNR
KGQFSIRINKQWRICFKWINNEPTEVEIVDYH

Nucleotide

• Length: 339 bp

>T59170 NZ_CP013667:849775-850113 [Coxiella burnetii]
TTGCCTCTTACGCTGTATATTATGCTTGATGTAACGCGTAAAACTGTTATCCTTGAAGTGATAATAAAAAGTTTCAAGGA
CAAATACACTAAATATTTATATAAAGGTGTATCCGTTTCAAAATGGCAAGCCATACGAAAGCAGGCGGAAAGACGGCTTC
AGATTTTGGATTCAGTGACATCGTTAGATGATTTGAGAAGTTTGCCAAGCAATCGTTTTGAATCGTTGCGGGGGAATAGA
AAAGGACAATTTAGTATAAGGATTAATAAGCAATGGCGCATTTGTTTTAAGTGGATTAACAATGAGCCAACTGAAGTTGA
AATTGTGGATTATCATTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11458.39 Da
• Isoelectric Point: 10.1699

>AT59170 WP_010958263.1 NZ_CP013667:850119-850430 [Coxiella burnetii]
MKMAANRMRPIHPGEILAEELGFLDKMSANQLAKHLAIPTNRVTAILNGARSITADTALRLAKFFGTTPEFWLNLQDAYD
IKMALKKSGKKIEKEVTPYDQAA

Nucleotide

• Length: 312 bp

>AT59170 NZ_CP013667:850119-850430 [Coxiella burnetii]
ATGAAAATGGCAGCTAATAGAATGCGACCCATACATCCAGGTGAGATCCTTGCTGAAGAGTTGGGGTTTTTAGATAAGAT
GTCAGCTAATCAGTTAGCTAAACATTTAGCAATTCCGACCAACCGAGTGACCGCCATTCTCAATGGCGCGCGATCCATTA
CGGCGGATACCGCGCTACGATTGGCTAAGTTTTTTGGGACGACGCCGGAATTTTGGCTAAATCTCCAAGACGCCTATGAC
ATAAAAATGGCATTGAAAAAGTCCGGCAAAAAAATCGAAAAAGAAGTAACCCCTTACGACCAAGCCGCCTAG

Structures

Toxin

• AlphaFold DB: Q83BL3