Detailed information of TA system TA059162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4637473..4638075
• Replicon: chromosome
• Accession: NZ_CP013663
• Organism: Escherichia coli strain GB089

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CO57_RS24875
• Protein ID: WP_000897305.1
• Coordinates: 4637473..4637784 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CO57_RS24880
• Protein ID: WP_000356397.1
• Coordinates: 4637785..4638075 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO57_RS24845	4632503..4633288	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
CO57_RS24850	4633387..4633986	+	600	WP_001315111.1	glucose-1-phosphatase	-
CO57_RS24855	4633980..4634852	+	873	WP_000920762.1	virulence factor BrkB family protein	-
CO57_RS24860	4634849..4635286	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CO57_RS24865	4635331..4636272	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CO57_RS24870	4636336..4637244	-	909	WP_001162704.1	alpha/beta hydrolase	-
CO57_RS24875	4637473..4637784	+	312	WP_000897305.1	hypothetical protein	Toxin
CO57_RS24880	4637785..4638075	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CO57_RS24885	4638680..4638898	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CO57_RS24890	4639117..4639359	+	243	WP_001086388.1	hypothetical protein	-
CO57_RS24900	4639689..4640618	-	930	WP_001749031.1	formate dehydrogenase accessory protein FdhE	-
CO57_RS24905	4640615..4641250	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CO57_RS24910	4641247..4642149	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T59162 WP_000897305.1 NZ_CP013663:4637473-4637784 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T59162 NZ_CP013663:4637473-4637784 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT59162 WP_000356397.1 NZ_CP013663:4637785-4638075 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT59162 NZ_CP013663:4637785-4638075 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7