Detailed information of TA system TA059141

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-OrzO/SymE(toxin)
• Location: 5063056..5063468
• Replicon: chromosome
• Accession: NZ_CP013662
• Organism: Escherichia coli strain 08-00022

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A0B1K116
• Locus tag: CP48_RS26890
• Protein ID: WP_000132623.1
• Coordinates: 5063056..5063397 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	OrzO
Locus tag	-
Coordinates	5063392..5063468 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP48_RS26870	5059305..5059469	+	165	WP_000394278.1	DUF1127 domain-containing protein	-
CP48_RS26875	5059567..5060439	+	873	WP_064760779.1	DUF262 domain-containing protein	-
CP48_RS26880	5060550..5061662	-	1113	WP_001300563.1	IS4-like element IS421 family transposase	-
CP48_RS30045	5061846..5063009	+	1164	WP_071940936.1	DUF1524 domain-containing protein	-
CP48_RS26890	5063056..5063397	-	342	WP_000132623.1	endoribonuclease SymE	Toxin
-	5063392..5063468	+	77	NuclAT_12	-	Antitoxin
-	5063392..5063468	+	77	NuclAT_12	-	Antitoxin
-	5063392..5063468	+	77	NuclAT_12	-	Antitoxin
-	5063392..5063468	+	77	NuclAT_12	-	Antitoxin
-	5063392..5063468	+	77	NuclAT_13	-	Antitoxin
-	5063392..5063468	+	77	NuclAT_13	-	Antitoxin
-	5063392..5063468	+	77	NuclAT_13	-	Antitoxin
-	5063392..5063468	+	77	NuclAT_13	-	Antitoxin
CP48_RS26895	5063612..5066875	-	3264	WP_000058876.1	type I restriction endonuclease subunit R	-
CP48_RS26900	5066970..5068367	-	1398	WP_000535016.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5059567..5068367	8800

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12323.21 Da
• Isoelectric Point: 9.5818

>T59141 WP_000132623.1 NZ_CP013662:c5063397-5063056 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMKGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVREFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T59141 NZ_CP013662:c5063397-5063056 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTATCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCTA
CCGGCACGGCGGTAGATGTCAAAGTGATGAAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGAGCGAG
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCGGGAATTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT59141 NZ_CP013662:5063392-5063468 [Escherichia coli]
AGTCATAACTGCTATTCTCCGATAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0B1K116

Antitoxin