Detailed information of TA system TA059093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1333814..1334316
• Replicon: chromosome
• Accession: NZ_CP013658
• Organism: Escherichia coli strain uk_P46212

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: AUO99_RS08095
• Protein ID: WP_000767819.1
• Coordinates: 1333814..1334068 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: AUO99_RS08100
• Protein ID: WP_001259253.1
• Coordinates: 1334065..1334316 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AUO99_RS08070	1329632..1330090	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
AUO99_RS08080	1330307..1331665	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
AUO99_RS29230	1331655..1331717	-	63	WP_010723108.1	membrane protein YoeI	-
AUO99_RS08085	1331932..1332861	-	930	WP_000803366.1	LysR family transcriptional regulator	-
AUO99_RS08090	1332907..1333731	-	825	WP_000754737.1	SDR family oxidoreductase	-
AUO99_RS08095	1333814..1334068	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
AUO99_RS08100	1334065..1334316	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
AUO99_RS27015	1334599..1334649	+	51	WP_001364200.1	his operon leader peptide	-
AUO99_RS08105	1334795..1335694	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
AUO99_RS08110	1335700..1337004	+	1305	WP_001296211.1	histidinol dehydrogenase	-
AUO99_RS08115	1337001..1338071	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
AUO99_RS08120	1338071..1339138	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1329632..1330090	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T59093 WP_000767819.1 NZ_CP013658:c1334068-1333814 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T59093 NZ_CP013658:c1334068-1333814 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT59093 WP_001259253.1 NZ_CP013658:c1334316-1334065 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT59093 NZ_CP013658:c1334316-1334065 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3