Detailed information of TA system TA059076

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 70017..70286
• Replicon: plasmid unnamed
• Accession: NZ_CP013657
• Organism: Escherichia coli strain uk_P46212

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AUO99_RS00425
• Protein ID: WP_001312861.1
• Coordinates: 70128..70286 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	70017..70082 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AUO99_RS00395	65852..66337	+	486	WP_042029316.1	single-stranded DNA-binding protein	-
AUO99_RS00400	66393..66626	+	234	WP_000006004.1	DUF905 domain-containing protein	-
AUO99_RS00405	66685..68643	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
AUO99_RS00410	68698..69132	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
AUO99_RS00415	69129..69848	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
AUO99_RS29570	69860..70048	-	189	WP_001299721.1	hypothetical protein	-
-	69860..70084	+	225	NuclAT_0	-	-
-	69860..70084	+	225	NuclAT_0	-	-
-	69860..70084	+	225	NuclAT_0	-	-
-	69860..70084	+	225	NuclAT_0	-	-
-	70017..70082	+	66	-	-	Antitoxin
AUO99_RS00425	70128..70286	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
AUO99_RS00435	71205..71492	+	288	WP_000107537.1	hypothetical protein	-
AUO99_RS00440	71612..72433	+	822	WP_001234469.1	DUF945 domain-containing protein	-
AUO99_RS00445	72730..73377	-	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
AUO99_RS00450	73663..74046	+	384	WP_001151564.1	relaxosome protein TraM	-
AUO99_RS00455	74240..74926	+	687	WP_000332487.1	PAS domain-containing protein	-
AUO99_RS00460	75020..75247	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / blaTEM-1B / tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / aadA5 / qacE / sul1	-	1..143748	143748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T59076 WP_001312861.1 NZ_CP013657:70128-70286 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T59076 NZ_CP013657:70128-70286 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT59076 NZ_CP013657:70017-70082 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin