Detailed information of TA system TA059065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2649231..2649829
• Replicon: chromosome
• Accession: NZ_CP013650
• Organism: Lacimicrobium alkaliphilum strain YelD216

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AT746_RS11995
• Protein ID: WP_062480620.1
• Coordinates: 2649506..2649829 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0U2JJ61
• Locus tag: AT746_RS11990
• Protein ID: WP_062480618.1
• Coordinates: 2649231..2649509 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AT746_RS11980	2646106..2647824	+	1719	WP_062480616.1	hypothetical protein	-
AT746_RS11985	2648169..2649166	+	998	Protein_2350	SEC-C domain-containing protein	-
AT746_RS11990	2649231..2649509	-	279	WP_062480618.1	helix-turn-helix domain-containing protein	Antitoxin
AT746_RS11995	2649506..2649829	-	324	WP_062480620.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AT746_RS12000	2650145..2650666	-	522	WP_062480622.1	PEP-CTERM sorting domain-containing protein	-
AT746_RS12010	2651797..2652390	-	594	WP_062480624.1	dCTP deaminase	-
AT746_RS12015	2652682..2653746	-	1065	WP_062480626.1	iron-sulfur cluster carrier protein ApbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-85)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12416.68 Da
• Isoelectric Point: 10.5532

>T59065 WP_062480620.1 NZ_CP013650:c2649829-2649506 [Lacimicrobium alkaliphilum]
MTWKVDFYDGVEDIILRMPPKIQARMIKLLELMEKHGANLGPPHTESMGHGLFEIRAKAKEGIGRSLFCYLTGKRIYVLH
AFVKKSRKTPKHELELARQRQKEVNKS

Nucleotide

• Length: 324 bp

>T59065 NZ_CP013650:c2649829-2649506 [Lacimicrobium alkaliphilum]
ATGACTTGGAAAGTCGATTTTTACGATGGCGTTGAAGATATCATTTTGCGAATGCCACCAAAGATCCAGGCAAGAATGAT
TAAATTACTGGAACTGATGGAAAAGCACGGGGCAAATTTAGGCCCACCTCATACTGAATCGATGGGGCATGGTCTGTTCG
AAATAAGAGCAAAAGCTAAGGAAGGGATTGGTCGCAGCCTGTTTTGCTACTTAACAGGCAAACGCATTTATGTATTACAT
GCTTTCGTGAAAAAATCACGGAAAACCCCTAAACATGAACTTGAGCTGGCCCGTCAACGCCAGAAAGAGGTAAACAAATC
ATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10498.02 Da
• Isoelectric Point: 8.0034

>AT59065 WP_062480618.1 NZ_CP013650:c2649509-2649231 [Lacimicrobium alkaliphilum]
MSLQNLKQRALDNPEVKAEYDDLESEFNLIDKLLTMRTTAGLTQEQVAERMKTQKSNISRLERGNANPSWATLLKYAHAC
GFDLSLKMQKIP

Nucleotide

• Length: 279 bp

>AT59065 NZ_CP013650:c2649509-2649231 [Lacimicrobium alkaliphilum]
ATGAGCTTACAAAATCTGAAACAGCGTGCACTCGATAATCCCGAGGTTAAGGCTGAATATGACGACCTGGAGTCTGAATT
TAATTTAATTGATAAGTTGCTCACGATGCGCACCACTGCAGGGCTGACGCAAGAGCAGGTGGCTGAGCGAATGAAAACGC
AAAAGAGCAATATCAGTCGTCTGGAGCGTGGCAATGCCAACCCCAGTTGGGCGACCCTGCTCAAATATGCCCATGCTTGT
GGTTTTGATTTGTCACTGAAAATGCAAAAAATACCCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0U2JJ61