Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2529278..2529888 Replicon chromosome
Accession NZ_CP013650
Organism Lacimicrobium alkaliphilum strain YelD216

Toxin (Protein)


Gene name graT Uniprot ID A0A0U2Z7D3
Locus tag AT746_RS11525 Protein ID WP_062480443.1
Coordinates 2529607..2529888 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID A0A0U3ALJ3
Locus tag AT746_RS11520 Protein ID WP_062480442.1
Coordinates 2529278..2529592 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AT746_RS11490 2524868..2525389 + 522 WP_062480432.1 DUF2062 domain-containing protein -
AT746_RS11495 2525599..2526480 - 882 WP_062480434.1 NmrA family NAD(P)-binding protein -
AT746_RS11500 2526610..2527503 + 894 WP_062480436.1 LysR family transcriptional regulator -
AT746_RS11505 2527786..2528286 + 501 WP_062480438.1 hypothetical protein -
AT746_RS11510 2528279..2528491 + 213 WP_062480440.1 helix-turn-helix transcriptional regulator -
AT746_RS11515 2528708..2529130 + 423 WP_082633246.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
AT746_RS11520 2529278..2529592 - 315 WP_062480442.1 HigA family addiction module antidote protein Antitoxin
AT746_RS11525 2529607..2529888 - 282 WP_062480443.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AT746_RS11530 2530022..2530387 - 366 WP_062480449.1 hypothetical protein -
AT746_RS11540 2531054..2531284 + 231 WP_062480454.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
AT746_RS11545 2531281..2531616 + 336 WP_062480455.1 endoribonuclease MazF -
AT746_RS11550 2531884..2534343 - 2460 WP_062480457.1 SIR2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-93)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(10-102)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10721.15 Da        Isoelectric Point: 7.8770

>T59062 WP_062480443.1 NZ_CP013650:c2529888-2529607 [Lacimicrobium alkaliphilum]
MIKSFADKRTQELYSKGKSKKFPADVAPRAARKLEYVDLAVQLEDLKVPPGNRLHPLTGNRQGQHAISINDQWRICFRFE
DGDAYEVEVCDYH

Download         Length: 282 bp

>T59062 NZ_CP013650:c2529888-2529607 [Lacimicrobium alkaliphilum]
ATGATCAAATCCTTCGCTGACAAACGAACCCAAGAACTGTACTCCAAAGGTAAGTCAAAGAAATTTCCTGCGGATGTTGC
ACCGAGAGCCGCCAGAAAACTCGAGTACGTGGATCTGGCTGTGCAGCTGGAGGATTTAAAGGTGCCGCCCGGCAATCGCC
TTCACCCGCTGACGGGAAACAGGCAGGGGCAGCACGCAATATCAATAAATGATCAGTGGCGTATCTGTTTTCGTTTTGAA
GATGGTGATGCGTACGAGGTCGAAGTGTGTGACTACCACTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11606.37 Da        Isoelectric Point: 9.9896

>AT59062 WP_062480442.1 NZ_CP013650:c2529592-2529278 [Lacimicrobium alkaliphilum]
MAILNTAGMQRKPTHPGEMLREDFLVDYGLTVAGLAKSLGVSRQSVNELLRERRAVSPEMALRLARLFGNSPEFWLNAQR
SVDLWTAAQSVKDEVERIKPLNAA

Download         Length: 315 bp

>AT59062 NZ_CP013650:c2529592-2529278 [Lacimicrobium alkaliphilum]
ATGGCTATTCTTAACACTGCCGGCATGCAGCGCAAACCGACTCATCCTGGTGAAATGCTCAGAGAAGATTTCCTGGTGGA
CTATGGCCTGACCGTTGCCGGGTTGGCTAAATCTCTGGGCGTCTCACGCCAGTCAGTTAACGAATTACTGCGCGAACGTC
GTGCTGTCAGTCCTGAAATGGCGCTCCGGCTTGCCCGTTTGTTTGGTAATTCGCCGGAATTCTGGCTGAACGCGCAGCGA
TCTGTCGATCTGTGGACTGCCGCTCAGTCAGTCAAGGATGAAGTGGAGCGGATCAAGCCACTGAATGCTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0U2Z7D3

Antitoxin

Source ID Structure
AlphaFold DB A0A0U3ALJ3

References