Detailed information of TA system TA059061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1444557..1445161
• Replicon: chromosome
• Accession: NZ_CP013650
• Organism: Lacimicrobium alkaliphilum strain YelD216

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AT746_RS06570
• Protein ID: WP_062478073.1
• Coordinates: 1444557..1444874 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0U2RL04
• Locus tag: AT746_RS06575
• Protein ID: WP_062478076.1
• Coordinates: 1444871..1445161 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AT746_RS06555	1440058..1441572	-	1515	WP_156413761.1	SpoVR family protein	-
AT746_RS06560	1441671..1443242	-	1572	WP_062478067.1	response regulator	-
AT746_RS06565	1443389..1444303	+	915	WP_062478070.1	pirin family protein	-
AT746_RS06570	1444557..1444874	+	318	WP_062478073.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AT746_RS06575	1444871..1445161	+	291	WP_062478076.1	helix-turn-helix domain-containing protein	Antitoxin
AT746_RS06580	1445446..1446114	+	669	WP_062478080.1	DUF1707 and DUF2154 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12235.19 Da
• Isoelectric Point: 9.3562

>T59061 WP_062478073.1 NZ_CP013650:1444557-1444874 [Lacimicrobium alkaliphilum]
MVIIETPVFTRLITELMSDDEYKDLQEALVNRPDMGALIRNSGGLRKIRWALEGRGKSGGVRIIYYWMTADDQLYMLLAY
PKNEKENLTDAQTKVLRQIVQRWSK

Nucleotide

• Length: 318 bp

>T59061 NZ_CP013650:1444557-1444874 [Lacimicrobium alkaliphilum]
ATGGTAATCATAGAAACCCCCGTTTTCACAAGGCTTATCACCGAGTTAATGAGTGATGACGAATACAAGGACTTGCAGGA
AGCGCTGGTCAACCGTCCGGATATGGGAGCCTTGATCAGAAACAGTGGCGGTCTGCGAAAGATACGCTGGGCCTTGGAAG
GCCGGGGAAAAAGTGGCGGTGTACGGATCATTTACTACTGGATGACAGCCGATGATCAGCTATATATGCTGCTGGCGTAC
CCCAAGAATGAGAAAGAAAATTTAACTGATGCGCAAACCAAGGTGCTCAGACAGATAGTCCAAAGGTGGTCGAAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10753.25 Da
• Isoelectric Point: 7.3218

>AT59061 WP_062478076.1 NZ_CP013650:1444871-1445161 [Lacimicrobium alkaliphilum]
MNEEQFNALLESVRQADEIIQGKAKAARVLEFAEPEVKSIRAKTGLTQERFANVLGVSKRTLENWEQGRRHPTGPARALL
KILDADPIHALEALNH

Nucleotide

• Length: 291 bp

>AT59061 NZ_CP013650:1444871-1445161 [Lacimicrobium alkaliphilum]
ATGAATGAAGAACAGTTTAACGCCTTGCTGGAAAGTGTCCGACAGGCGGATGAAATTATCCAGGGAAAGGCTAAAGCTGC
CCGGGTGCTCGAGTTTGCTGAACCAGAAGTAAAGTCCATCCGCGCCAAGACCGGGCTGACTCAGGAGCGCTTCGCCAATG
TATTAGGGGTGAGCAAACGCACTCTGGAAAACTGGGAGCAGGGACGGCGCCATCCGACCGGGCCTGCCCGCGCGCTGCTT
AAAATACTGGACGCTGATCCCATACATGCACTGGAAGCTCTGAATCATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0U2RL04