Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 61491..62016 Replicon plasmid pRphaN161c
Accession NZ_CP013588
Organism Rhizobium phaseoli strain N161

Toxin (Protein)


Gene name yoeB Uniprot ID B3Q138
Locus tag AMC78_RS25520 Protein ID WP_010025739.1
Coordinates 61744..62016 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A192TIM2
Locus tag AMC78_RS25515 Protein ID WP_010025738.1
Coordinates 61491..61742 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC78_RS25505 58229..59572 - 1344 WP_064836828.1 adenylate/guanylate cyclase domain-containing protein -
AMC78_RS25510 60074..61411 + 1338 WP_064836830.1 nucleotide sugar dehydrogenase -
AMC78_RS25515 61491..61742 + 252 WP_010025738.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
AMC78_RS25520 61744..62016 + 273 WP_010025739.1 Txe/YoeB family addiction module toxin Toxin
AMC78_RS25525 62054..63229 - 1176 WP_012490438.1 DUF3095 domain-containing protein -
AMC78_RS25530 63352..63831 + 480 WP_064832083.1 class I SAM-dependent methyltransferase -
AMC78_RS33685 63902..64438 + 537 WP_150126763.1 hypothetical protein -
AMC78_RS25535 64516..66429 - 1914 WP_064836832.1 cyclase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..407297 407297


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10743.42 Da        Isoelectric Point: 9.4530

>T58988 WP_010025739.1 NZ_CP013588:61744-62016 [Rhizobium phaseoli]
MKLLWTPTSWEEYQYWQKTDQKMVEKINELLKDTKRSPFKGLGKPEPLKGDLSGLWSRRILGEHRLVYCVTGKGSDQQLE
IIQCRFHYEK

Download         Length: 273 bp

>T58988 NZ_CP013588:61744-62016 [Rhizobium phaseoli]
ATGAAGCTGCTATGGACCCCGACTTCCTGGGAAGAATACCAATACTGGCAGAAAACCGACCAGAAGATGGTCGAGAAAAT
CAACGAACTGCTCAAGGATACAAAAAGATCGCCCTTTAAAGGTCTCGGCAAGCCGGAACCGCTGAAGGGCGATCTATCCG
GACTTTGGTCCCGGCGTATCCTCGGAGAGCATCGACTCGTCTACTGCGTTACTGGCAAGGGTTCAGACCAGCAGTTGGAA
ATTATCCAATGCCGATTTCATTACGAAAAATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9345.63 Da        Isoelectric Point: 5.6513

>AT58988 WP_010025738.1 NZ_CP013588:61491-61742 [Rhizobium phaseoli]
MAHVRLTEFRQNIATYFDQVLSSRAPLLVTRQGSEAMVLIAESEYESMQETLHLLSTPANSARLRESIAQLRKGQVIESD
PTL

Download         Length: 252 bp

>AT58988 NZ_CP013588:61491-61742 [Rhizobium phaseoli]
ATGGCACACGTCCGATTGACGGAGTTCCGGCAGAACATCGCAACCTATTTTGATCAGGTCCTGTCTTCTCGGGCTCCTCT
GCTCGTGACTCGTCAAGGTTCGGAAGCAATGGTCTTGATTGCCGAAAGCGAGTACGAAAGCATGCAAGAGACGCTGCACC
TGCTCTCGACGCCTGCCAATTCCGCCCGCTTGCGTGAAAGCATCGCACAGCTTCGCAAGGGCCAGGTGATAGAGAGCGAC
CCGACTCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192TIV7

Antitoxin

Source ID Structure
AlphaFold DB A0A192TIM2

References