Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 850347..850958 Replicon plasmid pRphaN771e
Accession NZ_CP013573
Organism Rhizobium phaseoli strain N771

Toxin (Protein)


Gene name graT Uniprot ID A0A192TNT1
Locus tag AMC81_RS34245 Protein ID WP_037113455.1
Coordinates 850677..850958 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A246TMY6
Locus tag AMC81_RS31440 Protein ID WP_037144436.1
Coordinates 850347..850646 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC81_RS31420 845601..847103 + 1503 WP_037144428.1 sugar ABC transporter ATP-binding protein -
AMC81_RS31425 847100..848110 + 1011 WP_037144430.1 ABC transporter permease -
AMC81_RS31430 848151..849128 + 978 WP_037144432.1 substrate-binding domain-containing protein -
AMC81_RS31435 849193..850086 + 894 WP_004670117.1 amidohydrolase -
AMC81_RS31440 850347..850646 - 300 WP_037144436.1 HigA family addiction module antidote protein Antitoxin
AMC81_RS34245 850677..850958 - 282 WP_037113455.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AMC81_RS31445 851467..851757 + 291 WP_037113457.1 hypothetical protein -
AMC81_RS31450 852316..854535 - 2220 WP_004670160.1 methyl-accepting chemotaxis protein -
AMC81_RS31455 854747..855655 - 909 WP_037113460.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - gmd 1..1189373 1189373


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-80)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10455.94 Da        Isoelectric Point: 9.1204

>T58966 WP_037113455.1 NZ_CP013573:c850958-850677 [Rhizobium phaseoli]
VIRSFKDAKTEAVARGKAPKGFPSDIVRSAIRKLTILNSVAVLEDLRSPPGNRLEALKGNREGQHSIRINDQWRICFVWT
DAGIEDVEIVDYH

Download         Length: 282 bp

>T58966 NZ_CP013573:c850958-850677 [Rhizobium phaseoli]
GTGATCAGGTCATTCAAGGACGCCAAAACCGAAGCCGTCGCGCGGGGCAAAGCACCAAAAGGATTTCCTTCTGACATCGT
GAGGTCTGCCATTCGGAAATTGACGATCCTGAACAGCGTTGCCGTGCTTGAGGACCTGCGTTCTCCTCCGGGAAATCGAT
TAGAAGCCCTTAAGGGCAATAGGGAAGGCCAGCATTCGATCCGGATCAATGATCAGTGGCGAATCTGTTTCGTATGGACA
GATGCCGGTATCGAAGACGTCGAAATAGTTGACTACCATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11344.19 Da        Isoelectric Point: 6.2263

>AT58966 WP_037144436.1 NZ_CP013573:c850646-850347 [Rhizobium phaseoli]
MTHILPPVHPGEILREEYLLPLNMSAGALAKKLNVPRTRIERIAAEQTPITTDTALRLAKYFRTTPEFWMNMQTSFNLKT
EEMALHSELELIPEIDRAA

Download         Length: 300 bp

>AT58966 NZ_CP013573:c850646-850347 [Rhizobium phaseoli]
ATGACCCATATACTTCCGCCGGTCCATCCGGGCGAAATTCTCCGCGAGGAATACCTACTTCCTCTGAACATGAGCGCCGG
CGCACTGGCGAAAAAGCTTAATGTTCCTCGCACGCGCATCGAACGTATAGCGGCGGAGCAAACACCGATTACGACGGATA
CCGCACTGCGCCTTGCAAAGTACTTCCGCACAACGCCGGAATTTTGGATGAACATGCAGACAAGCTTCAACCTCAAGACA
GAAGAAATGGCACTTCATAGCGAATTGGAGCTTATCCCGGAAATCGATAGAGCGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192TNT1

Antitoxin

Source ID Structure
AlphaFold DB A0A246TMY6

References