Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 62060..62585 Replicon plasmid pRphaN931b
Accession NZ_CP013554
Organism Rhizobium phaseoli strain N931

Toxin (Protein)


Gene name yoeB Uniprot ID B3Q138
Locus tag AMC84_RS22800 Protein ID WP_010025739.1
Coordinates 62313..62585 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A192TIM2
Locus tag AMC84_RS22795 Protein ID WP_010025738.1
Coordinates 62060..62311 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC84_RS22785 58467..59810 - 1344 WP_004679763.1 adenylate/guanylate cyclase domain-containing protein -
AMC84_RS22790 60643..61980 + 1338 WP_064823466.1 nucleotide sugar dehydrogenase -
AMC84_RS22795 62060..62311 + 252 WP_010025738.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
AMC84_RS22800 62313..62585 + 273 WP_010025739.1 Txe/YoeB family addiction module toxin Toxin
AMC84_RS22805 62623..63798 - 1176 WP_012490438.1 DUF3095 domain-containing protein -
AMC84_RS22810 63921..64400 + 480 WP_064823467.1 class I SAM-dependent methyltransferase -
AMC84_RS33585 64471..65007 + 537 WP_150124917.1 hypothetical protein -
AMC84_RS22815 65085..66998 - 1914 WP_064823468.1 cyclase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..431484 431484


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10743.42 Da        Isoelectric Point: 9.4530

>T58938 WP_010025739.1 NZ_CP013554:62313-62585 [Rhizobium phaseoli]
MKLLWTPTSWEEYQYWQKTDQKMVEKINELLKDTKRSPFKGLGKPEPLKGDLSGLWSRRILGEHRLVYCVTGKGSDQQLE
IIQCRFHYEK

Download         Length: 273 bp

>T58938 NZ_CP013554:62313-62585 [Rhizobium phaseoli]
ATGAAGCTGCTATGGACCCCGACTTCCTGGGAAGAATACCAATACTGGCAGAAAACCGACCAGAAGATGGTCGAGAAAAT
CAACGAACTGCTCAAGGATACAAAAAGATCGCCCTTTAAAGGTCTCGGCAAGCCGGAACCGCTGAAGGGCGATCTATCCG
GACTTTGGTCCCGGCGTATCCTCGGAGAGCATCGACTCGTCTACTGCGTTACTGGCAAGGGTTCAGACCAGCAGTTGGAA
ATCATCCAATGCCGATTTCATTACGAAAAATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9345.63 Da        Isoelectric Point: 5.6513

>AT58938 WP_010025738.1 NZ_CP013554:62060-62311 [Rhizobium phaseoli]
MAHVRLTEFRQNIATYFDQVLSSRAPLLVTRQGSEAMVLIAESEYESMQETLHLLSTPANSARLRESIAQLRKGQVIESD
PTL

Download         Length: 252 bp

>AT58938 NZ_CP013554:62060-62311 [Rhizobium phaseoli]
ATGGCACACGTCCGATTGACGGAGTTCCGGCAGAACATCGCAACCTATTTTGATCAGGTCCTGTCTTCTCGGGCTCCTCT
GCTCGTGACCCGTCAAGGTTCGGAAGCAATGGTCTTGATTGCCGAAAGCGAGTACGAAAGCATGCAAGAGACGCTGCACC
TGCTCTCGACGCCTGCCAATTCCGCCCGCTTGCGTGAAAGCATCGCACAGCTTCGCAAGGGCCAGGTGATAGAGAGCGAC
CCGACTCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192TIV7

Antitoxin

Source ID Structure
AlphaFold DB A0A192TIM2

References