Detailed information of TA system TA058930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 66686..67211
• Replicon: plasmid pRetR611b
• Accession: NZ_CP013549
• Organism: Rhizobium phaseoli strain R611

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: B3Q138
• Locus tag: AMC85_RS23650
• Protein ID: WP_010025739.1
• Coordinates: 66939..67211 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A192TIM2
• Locus tag: AMC85_RS23645
• Protein ID: WP_010025738.1
• Coordinates: 66686..66937 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC85_RS23635	63424..64767	-	1344	WP_064826145.1	adenylate/guanylate cyclase domain-containing protein	-
AMC85_RS23640	65269..66606	+	1338	WP_010025737.1	nucleotide sugar dehydrogenase	-
AMC85_RS23645	66686..66937	+	252	WP_010025738.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
AMC85_RS23650	66939..67211	+	273	WP_010025739.1	Txe/YoeB family addiction module toxin	Toxin
AMC85_RS23655	67249..68424	-	1176	WP_012490438.1	DUF3095 domain-containing protein	-
AMC85_RS23660	68547..69026	+	480	WP_010024037.1	class I SAM-dependent methyltransferase	-
AMC85_RS33150	69094..69582	+	489	WP_150128066.1	hypothetical protein	-
AMC85_RS23665	69693..71624	-	1932	WP_064826146.1	cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..413457	413457

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10743.42 Da
• Isoelectric Point: 9.4530

>T58930 WP_010025739.1 NZ_CP013549:66939-67211 [Rhizobium phaseoli]
MKLLWTPTSWEEYQYWQKTDQKMVEKINELLKDTKRSPFKGLGKPEPLKGDLSGLWSRRILGEHRLVYCVTGKGSDQQLE
IIQCRFHYEK

Nucleotide

• Length: 273 bp

>T58930 NZ_CP013549:66939-67211 [Rhizobium phaseoli]
ATGAAGCTGCTATGGACCCCGACTTCCTGGGAAGAATACCAATACTGGCAGAAAACCGACCAGAAGATGGTCGAGAAAAT
CAACGAACTGCTCAAGGATACAAAAAGATCGCCCTTTAAAGGTCTCGGCAAGCCGGAACCGCTGAAGGGCGATCTATCCG
GACTTTGGTCCCGGCGTATCCTCGGAGAGCATCGACTCGTCTACTGCGTTACTGGCAAGGGTTCAGACCAGCAGTTGGAA
ATCATCCAATGCCGATTTCATTACGAAAAATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9345.63 Da
• Isoelectric Point: 5.6513

>AT58930 WP_010025738.1 NZ_CP013549:66686-66937 [Rhizobium phaseoli]
MAHVRLTEFRQNIATYFDQVLSSRAPLLVTRQGSEAMVLIAESEYESMQETLHLLSTPANSARLRESIAQLRKGQVIESD
PTL

Nucleotide

• Length: 252 bp

>AT58930 NZ_CP013549:66686-66937 [Rhizobium phaseoli]
ATGGCACACGTCCGATTGACGGAGTTCCGGCAGAACATCGCAACCTATTTTGATCAGGTCCTGTCTTCTCGGGCTCCTCT
GCTCGTGACTCGTCAAGGTTCGGAAGCAATGGTCTTGATTGCCGAAAGCGAGTACGAAAGCATGCAAGAGACGCTGCACC
TGCTCTCGACGCCTGCCAATTCCGCCCGCTTGCGTGAAAGCATCGCACAGCTTCGCAAGGGCCAGGTGATAGAGAGCGAC
CCGACTCTCTGA

Structures

Toxin

• AlphaFold DB: A0A192TIV7

Antitoxin

• AlphaFold DB: A0A192TIM2