Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 821544..822155 Replicon plasmid pRphaR723d
Accession NZ_CP013531
Organism Rhizobium phaseoli strain R723

Toxin (Protein)


Gene name graT Uniprot ID A0A192TNT1
Locus tag AMC89_RS33150 Protein ID WP_037113455.1
Coordinates 821544..821825 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A246TMY6
Locus tag AMC89_RS30705 Protein ID WP_037144436.1
Coordinates 821856..822155 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC89_RS30685 817102..818511 - 1410 WP_064820400.1 aldehyde dehydrogenase family protein -
AMC89_RS30690 818508..819572 - 1065 WP_064820401.1 homoserine dehydrogenase -
AMC89_RS30695 819806..820273 + 468 WP_004670133.1 Lrp/AsnC family transcriptional regulator -
AMC89_RS30700 820745..821035 - 291 WP_037113457.1 hypothetical protein -
AMC89_RS33150 821544..821825 + 282 WP_037113455.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AMC89_RS30705 821856..822155 + 300 WP_037144436.1 HigA family addiction module antidote protein Antitoxin
AMC89_RS30710 822416..823309 - 894 WP_004670117.1 amidohydrolase -
AMC89_RS30715 823374..824351 - 978 WP_064820402.1 substrate-binding domain-containing protein -
AMC89_RS30720 824392..825402 - 1011 WP_037113453.1 ABC transporter permease -
AMC89_RS30725 825399..826901 - 1503 WP_004670111.1 sugar ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - gmd 1..1074795 1074795


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-80)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10455.94 Da        Isoelectric Point: 9.1204

>T58896 WP_037113455.1 NZ_CP013531:821544-821825 [Rhizobium phaseoli]
VIRSFKDAKTEAVARGKAPKGFPSDIVRSAIRKLTILNSVAVLEDLRSPPGNRLEALKGNREGQHSIRINDQWRICFVWT
DAGIEDVEIVDYH

Download         Length: 282 bp

>T58896 NZ_CP013531:821544-821825 [Rhizobium phaseoli]
GTGATCAGGTCATTCAAGGACGCCAAAACCGAAGCCGTCGCGCGGGGCAAAGCACCAAAAGGATTTCCTTCTGACATCGT
GAGGTCTGCCATTCGGAAATTGACGATCCTGAACAGCGTTGCCGTGCTTGAGGACCTGCGTTCTCCTCCGGGAAATCGAT
TAGAAGCCCTTAAGGGCAATAGGGAAGGCCAGCATTCGATCCGGATCAATGATCAGTGGCGAATCTGTTTCGTATGGACA
GATGCCGGTATCGAAGACGTCGAAATAGTTGACTACCATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11344.19 Da        Isoelectric Point: 6.2263

>AT58896 WP_037144436.1 NZ_CP013531:821856-822155 [Rhizobium phaseoli]
MTHILPPVHPGEILREEYLLPLNMSAGALAKKLNVPRTRIERIAAEQTPITTDTALRLAKYFRTTPEFWMNMQTSFNLKT
EEMALHSELELIPEIDRAA

Download         Length: 300 bp

>AT58896 NZ_CP013531:821856-822155 [Rhizobium phaseoli]
ATGACCCATATACTTCCGCCGGTCCATCCGGGCGAAATTCTCCGCGAGGAATACCTACTTCCTCTGAACATGAGCGCCGG
CGCACTGGCGAAAAAGCTTAATGTTCCTCGCACGCGCATCGAACGTATAGCGGCGGAGCAAACACCGATTACGACGGATA
CCGCACTGCGCCTTGCAAAGTACTTCCGCACAACGCCGGAATTTTGGATGAACATGCAGACAAGCTTCAACCTCAAGACA
GAAGAAATGGCACTTCATAGCGAATTGGAGCTTATCCCGGAAATCGATAGAGCGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192TNT1

Antitoxin

Source ID Structure
AlphaFold DB A0A246TMY6

References