Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 61822..62347 Replicon plasmid pRphaR744c
Accession NZ_CP013525
Organism Rhizobium phaseoli strain R744

Toxin (Protein)


Gene name yoeB Uniprot ID B3Q138
Locus tag AMC90_RS24360 Protein ID WP_010025739.1
Coordinates 62075..62347 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A192TIM2
Locus tag AMC90_RS24355 Protein ID WP_010025738.1
Coordinates 61822..62073 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC90_RS24345 58560..59903 - 1344 WP_064826145.1 adenylate/guanylate cyclase domain-containing protein -
AMC90_RS24350 60405..61742 + 1338 WP_010025737.1 nucleotide sugar dehydrogenase -
AMC90_RS24355 61822..62073 + 252 WP_010025738.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
AMC90_RS24360 62075..62347 + 273 WP_010025739.1 Txe/YoeB family addiction module toxin Toxin
AMC90_RS24365 62385..63560 - 1176 WP_012490438.1 DUF3095 domain-containing protein -
AMC90_RS24370 63683..64162 + 480 WP_016736525.1 class I SAM-dependent methyltransferase -
AMC90_RS32920 64233..64718 + 486 WP_141397925.1 hypothetical protein -
AMC90_RS24375 64829..66760 - 1932 WP_064826146.1 cyclase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..407814 407814


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10743.42 Da        Isoelectric Point: 9.4530

>T58883 WP_010025739.1 NZ_CP013525:62075-62347 [Rhizobium phaseoli]
MKLLWTPTSWEEYQYWQKTDQKMVEKINELLKDTKRSPFKGLGKPEPLKGDLSGLWSRRILGEHRLVYCVTGKGSDQQLE
IIQCRFHYEK

Download         Length: 273 bp

>T58883 NZ_CP013525:62075-62347 [Rhizobium phaseoli]
ATGAAGCTGCTATGGACCCCGACTTCCTGGGAAGAATACCAATACTGGCAGAAAACCGACCAGAAGATGGTCGAGAAAAT
CAACGAACTGCTCAAGGATACAAAAAGATCGCCCTTTAAAGGTCTCGGCAAGCCGGAACCGCTGAAGGGCGATCTATCCG
GACTTTGGTCCCGGCGTATCCTCGGAGAGCATCGACTCGTCTACTGCGTTACTGGCAAGGGTTCAGACCAGCAGTTGGAA
ATCATCCAATGCCGATTTCATTACGAAAAATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9345.63 Da        Isoelectric Point: 5.6513

>AT58883 WP_010025738.1 NZ_CP013525:61822-62073 [Rhizobium phaseoli]
MAHVRLTEFRQNIATYFDQVLSSRAPLLVTRQGSEAMVLIAESEYESMQETLHLLSTPANSARLRESIAQLRKGQVIESD
PTL

Download         Length: 252 bp

>AT58883 NZ_CP013525:61822-62073 [Rhizobium phaseoli]
ATGGCACACGTCCGATTGACGGAGTTCCGGCAGAACATCGCAACCTATTTTGATCAGGTCCTGTCTTCTCGGGCTCCTCT
GCTCGTGACTCGTCAAGGTTCGGAAGCAATGGTCTTGATTGCCGAAAGCGAGTACGAAAGCATGCAAGAGACGCTGCACC
TGCTCTCGACGCCTGCCAATTCCGCCCGCTTGCGTGAAAGCATCGCACAGCTTCGCAAGGGCCAGGTGATAGAGAGCGAC
CCGACTCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192TIV7

Antitoxin

Source ID Structure
AlphaFold DB A0A192TIM2

References