Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2261661..2262163 Replicon chromosome
Accession NZ_CP013483
Organism Escherichia coli strain Y5

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ATO45_RS11035 Protein ID WP_000767829.1
Coordinates 2261909..2262163 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ATO45_RS11030 Protein ID WP_001259255.1
Coordinates 2261661..2261912 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ATO45_RS11010 2256839..2257906 - 1068 WP_000080090.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
ATO45_RS11015 2257906..2258976 - 1071 WP_000108933.1 histidinol-phosphate transaminase -
ATO45_RS11020 2258973..2260277 - 1305 WP_001446927.1 histidinol dehydrogenase -
ATO45_RS11025 2260283..2261182 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
ATO45_RS24530 2261328..2261378 - 51 WP_001364200.1 his operon leader peptide -
ATO45_RS11030 2261661..2261912 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ATO45_RS11035 2261909..2262163 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ATO45_RS11040 2262246..2263070 + 825 WP_000754737.1 SDR family oxidoreductase -
ATO45_RS11045 2263116..2264045 + 930 WP_000803351.1 LysR family transcriptional regulator -
ATO45_RS25675 2264260..2264322 + 63 WP_010723108.1 membrane protein YoeI -
ATO45_RS11050 2264312..2265670 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ATO45_RS11055 2265849..2266907 + 1059 WP_000492333.1 transport protein YeeE -
ATO45_RS11060 2266921..2267148 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T58819 WP_000767829.1 NZ_CP013483:2261909-2262163 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T58819 NZ_CP013483:2261909-2262163 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT58819 WP_001259255.1 NZ_CP013483:2261661-2261912 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT58819 NZ_CP013483:2261661-2261912 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References