Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 2357059..2357705 Replicon chromosome
Accession NZ_CP013480
Organism Pandoraea norimbergensis strain DSM 11628

Toxin (Protein)


Gene name graT Uniprot ID A0A0U2Y2U2
Locus tag AT302_RS10395 Protein ID WP_058378378.1
Coordinates 2357059..2357340 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0U2Y4B7
Locus tag AT302_RS10400 Protein ID WP_058378379.1
Coordinates 2357355..2357705 (+) Length 117 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AT302_RS10380 2352988..2353272 + 285 WP_087687838.1 acylphosphatase -
AT302_RS10385 2353352..2354995 - 1644 WP_058378376.1 Tar ligand binding domain-containing protein -
AT302_RS10390 2355266..2356993 + 1728 WP_058378377.1 response regulator -
AT302_RS10395 2357059..2357340 + 282 WP_058378378.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AT302_RS10400 2357355..2357705 + 351 WP_058378379.1 HigA family addiction module antidote protein Antitoxin
AT302_RS10405 2357729..2358382 - 654 WP_058378380.1 hemolysin III family protein -
AT302_RS10410 2358379..2358936 - 558 WP_058378381.1 peroxiredoxin -
AT302_RS10415 2359126..2359611 - 486 WP_087687832.1 Lrp/AsnC family transcriptional regulator -
AT302_RS10420 2359769..2361598 + 1830 WP_058378383.1 biosynthetic-type acetolactate synthase large subunit -
AT302_RS10425 2361755..2362531 + 777 WP_058380238.1 basic amino acid ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-85)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10677.51 Da        Isoelectric Point: 10.0074

>T58806 WP_058378378.1 NZ_CP013480:2357059-2357340 [Pandoraea norimbergensis]
MIQTFSDKVTAALFTGRYVRRFPKPIQRAALRKLLMIDAATCVEDLLVPLGNRLEGLHGKPVDLWSIRVNQQWRVCFRFA
KGDAYDVGIVDYH

Download         Length: 282 bp

>T58806 NZ_CP013480:2357059-2357340 [Pandoraea norimbergensis]
ATGATCCAGACCTTCTCGGACAAGGTGACCGCGGCACTCTTTACCGGTCGCTACGTACGCAGGTTTCCAAAACCGATTCA
GCGCGCCGCGTTACGCAAGTTATTGATGATCGATGCGGCGACTTGTGTGGAGGACTTGCTGGTGCCGCTGGGCAACCGAT
TGGAGGGTTTGCACGGTAAGCCAGTGGATTTGTGGAGCATTCGCGTCAATCAGCAATGGCGCGTGTGTTTCCGATTTGCC
AAGGGCGATGCCTACGACGTGGGTATCGTCGACTATCACTGA

Antitoxin


Download         Length: 117 a.a.        Molecular weight: 12540.42 Da        Isoelectric Point: 8.6594

>AT58806 WP_058378379.1 NZ_CP013480:2357355-2357705 [Pandoraea norimbergensis]
MSIRRDQIDQTDFAGLVTGQSLAPIHPGEILREEFMVPGGMSANALALALHVPAPRINDVVRGKRGISADTALRLARYFG
TSAEFWTNLQASYDLRVALQAAGADIIRQVLPLRAA

Download         Length: 351 bp

>AT58806 NZ_CP013480:2357355-2357705 [Pandoraea norimbergensis]
ATGAGCATCAGACGAGATCAGATCGACCAGACCGATTTTGCGGGGCTGGTCACGGGGCAGTCGCTGGCGCCGATTCATCC
GGGGGAAATTCTGCGCGAGGAATTCATGGTGCCGGGCGGCATGTCGGCGAACGCGCTGGCGTTGGCGCTTCATGTGCCGG
CACCGCGCATCAACGACGTCGTGCGCGGCAAGCGGGGTATTTCGGCGGATACCGCCCTGCGTCTTGCGCGCTACTTCGGC
ACCTCTGCGGAGTTCTGGACCAATCTCCAGGCGTCGTACGACCTGCGTGTTGCCTTGCAGGCCGCCGGCGCGGACATCAT
CCGGCAGGTGCTCCCGCTGCGTGCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0U2Y2U2

Antitoxin

Source ID Structure
AlphaFold DB A0A0U2Y4B7

References