Detailed information of TA system TA058799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5190688..5191283
• Replicon: chromosome
• Accession: NZ_CP013478
• Organism: Pseudomonas aeruginosa strain SCVJan

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: SCVJan_RS24590
• Protein ID: WP_003117425.1
• Coordinates: 5191005..5191283 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SCVJan_RS24585
• Protein ID: WP_003113527.1
• Coordinates: 5190688..5190993 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SCVJan_RS24550	5185828..5186676	+	849	WP_023103532.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
SCVJan_RS24560	5186843..5187784	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
SCVJan_RS24565	5187901..5188515	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
SCVJan_RS24570	5188557..5189141	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
SCVJan_RS24575	5189182..5190282	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
SCVJan_RS24585	5190688..5190993	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
SCVJan_RS24590	5191005..5191283	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SCVJan_RS24600	5191612..5193840	+	2229	WP_003113525.1	TonB-dependent receptor	-
SCVJan_RS24605	5193910..5194557	-	648	WP_003095021.1	carbonate dehydratase	-
SCVJan_RS24610	5194619..5195857	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T58799 WP_003117425.1 NZ_CP013478:c5191283-5191005 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T58799 NZ_CP013478:c5191283-5191005 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT58799 WP_003113527.1 NZ_CP013478:c5190993-5190688 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT58799 NZ_CP013478:c5190993-5190688 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5