Detailed information of TA system TA058714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 723831..724424
• Replicon: chromosome
• Accession: NZ_CP013464
• Organism: Burkholderia ubonensis strain MSMB1471WGS

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A124NDB4
• Locus tag: WM29_RS19370
• Protein ID: WP_042588736.1
• Coordinates: 724146..724424 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WM29_RS19365
• Protein ID: WP_088505171.1
• Coordinates: 723831..724133 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM29_RS19340	719080..720351	+	1272	WP_045564529.1	Rieske 2Fe-2S domain-containing protein	-
WM29_RS19345	720366..720842	+	477	WP_059618175.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
WM29_RS19350	720862..721188	+	327	WP_060304534.1	non-heme iron oxygenase ferredoxin subunit	-
WM29_RS19355	721178..722398	+	1221	WP_069237572.1	FAD-dependent oxidoreductase	-
WM29_RS19360	722489..723775	+	1287	WP_059592208.1	DUF445 family protein	-
WM29_RS19365	723831..724133	-	303	WP_088505171.1	HigA family addiction module antidote protein	Antitoxin
WM29_RS19370	724146..724424	-	279	WP_042588736.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WM29_RS19375	724796..725095	-	300	WP_060304541.1	putative addiction module antidote protein	-
WM29_RS19380	725099..725422	-	324	WP_069237573.1	type II toxin-antitoxin system RelE/ParE family toxin	-
WM29_RS19385	725865..727505	+	1641	WP_069237574.1	acetolactate synthase large subunit	-
WM29_RS19390	727535..728968	+	1434	WP_059967161.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-87)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10684.07 Da
• Isoelectric Point: 7.8922

>T58714 WP_042588736.1 NZ_CP013464:c724424-724146 [Burkholderia ubonensis]
MIRSFRCKDTQSLFAGESPKRFRTIESVATRKLEMVAAAKELRDLRSPPGNRLEALHGNRDGQHSIRINEQWRICFEWTD
DGPANVEILDYH

Nucleotide

• Length: 279 bp

>T58714 NZ_CP013464:c724424-724146 [Burkholderia ubonensis]
ATGATCAGATCGTTCCGATGCAAGGATACCCAGTCGCTTTTCGCCGGCGAATCACCCAAACGCTTCCGTACGATCGAATC
CGTTGCTACCCGGAAACTGGAAATGGTCGCTGCTGCGAAAGAACTGCGCGACTTGCGGTCTCCGCCCGGCAACCGGCTGG
AAGCGCTTCACGGAAATCGGGATGGCCAGCACAGCATCCGTATCAACGAGCAATGGCGGATCTGCTTCGAATGGACCGAC
GACGGCCCCGCCAACGTCGAGATCCTTGACTATCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11601.34 Da
• Isoelectric Point: 8.5562

>AT58714 WP_088505171.1 NZ_CP013464:c724133-723831 [Burkholderia ubonensis]
MLKNGMRPIHPGEILREEFLTPLEMSVNALALALNVPATRVHEIVKERRGVTADTAYRLARYFDTSPEFWLNLQATYDLK
TLPTRNEIDRKVEPRERTHA

Nucleotide

• Length: 303 bp

>AT58714 NZ_CP013464:c724133-723831 [Burkholderia ubonensis]
ATGCTCAAGAATGGAATGCGTCCGATCCACCCGGGCGAAATCCTGCGAGAAGAATTCCTGACGCCGCTGGAAATGAGTGT
CAACGCGCTTGCACTCGCGTTGAACGTACCGGCTACCCGCGTGCATGAAATCGTCAAGGAACGGCGCGGGGTCACGGCCG
ATACCGCTTACCGGCTCGCACGCTACTTCGACACGTCACCCGAATTCTGGCTGAACCTGCAAGCCACATACGACCTCAAA
ACGCTGCCGACGCGCAATGAGATCGACCGGAAGGTCGAACCGCGCGAACGCACGCACGCATAG

Structures

Toxin

• AlphaFold DB: A0A124NDB4