Detailed information of TA system TA058709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 204389..205055
• Replicon: chromosome
• Accession: NZ_CP013457
• Organism: Burkholderia sp. MSMB617WGS

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1B4S475
• Locus tag: WT60_RS01020
• Protein ID: WP_059645390.1
• Coordinates: 204389..204733 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WT60_RS01025
• Protein ID: WP_080471805.1
• Coordinates: 204714..205055 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WT60_RS00995	199565..200491	+	927	WP_060356268.1	AAA family ATPase	-
WT60_RS01000	200488..201852	+	1365	WP_060356269.1	type II secretory pathway protein	-
WT60_RS01010	202615..203394	+	780	WP_060356270.1	hypothetical protein	-
WT60_RS01015	203761..204348	+	588	WP_176373495.1	site-specific integrase	-
WT60_RS01020	204389..204733	+	345	WP_059645390.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WT60_RS01025	204714..205055	+	342	WP_080471805.1	helix-turn-helix domain-containing protein	Antitoxin
WT60_RS34255	205211..205570	-	360	WP_157130939.1	hypothetical protein	-
WT60_RS01035	205716..207269	-	1554	WP_059584212.1	IS66 family transposase	-
WT60_RS01040	207300..207644	-	345	WP_059584214.1	IS66 family insertion sequence element accessory protein TnpB	-
WT60_RS01045	207641..208045	-	405	WP_100185218.1	transposase	-
WT60_RS01050	208122..208460	+	339	Protein_184	integrase	-
WT60_RS01055	208461..209531	+	1071	WP_060356788.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	191433..217625	26192

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-102)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12986.09 Da
• Isoelectric Point: 10.3115

>T58709 WP_059645390.1 NZ_CP013457:204389-204733 [Burkholderia sp. MSMB617WGS]
MKQNWTVVYYNERVKRDVFALPAGILADYLRLLDLMQEFGADLRMPHSRAMGGGLFELRPKGREGIGRVLYCTHFGRHVV
VLHSFVKKAQEAPQKELRTARARLSEVRNGEGGC

Nucleotide

• Length: 345 bp

>T58709 NZ_CP013457:204389-204733 [Burkholderia sp. MSMB617WGS]
ATGAAACAAAATTGGACAGTCGTCTATTACAACGAGCGCGTTAAACGCGACGTCTTCGCGCTGCCGGCCGGGATTCTGGC
GGACTATCTGCGGCTCCTGGACTTGATGCAGGAGTTCGGCGCGGATTTGCGTATGCCGCACTCGCGAGCAATGGGGGGCG
GATTGTTCGAGTTGCGCCCGAAGGGGCGAGAGGGCATCGGGCGCGTGCTTTATTGCACGCACTTTGGGCGGCACGTCGTG
GTGCTGCATTCGTTTGTGAAGAAGGCGCAGGAGGCGCCGCAGAAAGAACTGCGCACCGCCCGAGCGCGTTTGAGCGAGGT
ACGAAATGGTGAAGGCGGTTGCTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12223.94 Da
• Isoelectric Point: 9.5971

>AT58709 WP_080471805.1 NZ_CP013457:204714-205055 [Burkholderia sp. MSMB617WGS]
MVKAVAKRVRAEGFSPVPHMTDDTERLLAKPGVKAQYDALEDEYSALRAILTARQDAGLTQAQVAERMGTTASAVSRLEA
SLSSERHSPSFATLRKYAAACGKRLVISFAENL

Nucleotide

• Length: 342 bp

>AT58709 NZ_CP013457:204714-205055 [Burkholderia sp. MSMB617WGS]
ATGGTGAAGGCGGTTGCTAAGCGCGTGCGCGCTGAAGGATTTAGTCCTGTTCCGCATATGACGGATGACACTGAGCGTCT
ACTGGCAAAGCCTGGTGTCAAAGCACAATACGATGCGCTAGAAGATGAATACAGCGCGCTTCGAGCCATCTTGACGGCTC
GGCAAGACGCCGGCTTGACGCAGGCTCAGGTGGCCGAGCGCATGGGAACGACAGCTTCTGCAGTTTCGCGATTGGAAGCG
TCACTGTCGAGCGAAAGGCACTCGCCGTCGTTCGCGACGTTGCGAAAGTATGCGGCAGCTTGTGGTAAGCGATTGGTGAT
CTCGTTTGCCGAGAATTTGTAA

Structures

Toxin

• AlphaFold DB: A0A1B4S475