Detailed information of TA system TA058636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 204559..205225
• Replicon: chromosome
• Accession: NZ_CP013424
• Organism: Burkholderia sp. MSMB0852

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1B4S475
• Locus tag: WS86_RS01410
• Protein ID: WP_059645390.1
• Coordinates: 204559..204903 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WS86_RS01415
• Protein ID: WP_080471805.1
• Coordinates: 204884..205225 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WS86_RS01385	199733..200659	+	927	WP_059645382.1	AAA family ATPase	-
WS86_RS01390	200656..202020	+	1365	WP_059645385.1	type II secretory pathway protein	-
WS86_RS01400	202785..203564	+	780	WP_059645387.1	hypothetical protein	-
WS86_RS01405	203931..204518	+	588	WP_176373495.1	site-specific integrase	-
WS86_RS01410	204559..204903	+	345	WP_059645390.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WS86_RS01415	204884..205225	+	342	WP_080471805.1	helix-turn-helix domain-containing protein	Antitoxin
WS86_RS33870	205381..205740	-	360	WP_157130939.1	hypothetical protein	-
WS86_RS01425	206120..206662	+	543	WP_082745147.1	PAAR domain-containing protein	-
WS86_RS35180	206659..207372	+	714	WP_198170015.1	VRR-NUC domain-containing protein	-
WS86_RS01435	207375..208451	+	1077	WP_059645396.1	DUF3396 domain-containing protein	-
WS86_RS01445	208919..209152	+	234	WP_059645603.1	hypothetical protein	-
WS86_RS01450	209327..209740	+	414	WP_082745137.1	DNA mismatch endonuclease Vsr	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	191609..219211	27602

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-102)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12986.09 Da
• Isoelectric Point: 10.3115

>T58636 WP_059645390.1 NZ_CP013424:204559-204903 [Burkholderia sp. MSMB0852]
MKQNWTVVYYNERVKRDVFALPAGILADYLRLLDLMQEFGADLRMPHSRAMGGGLFELRPKGREGIGRVLYCTHFGRHVV
VLHSFVKKAQEAPQKELRTARARLSEVRNGEGGC

Nucleotide

• Length: 345 bp

>T58636 NZ_CP013424:204559-204903 [Burkholderia sp. MSMB0852]
ATGAAACAAAATTGGACAGTCGTCTATTACAACGAGCGCGTTAAACGCGACGTCTTCGCGCTGCCGGCCGGGATTCTGGC
GGACTATCTGCGGCTCCTGGACTTGATGCAGGAGTTCGGCGCGGATTTGCGTATGCCGCACTCGCGAGCAATGGGGGGCG
GATTGTTCGAGTTGCGCCCGAAGGGGCGAGAGGGCATCGGGCGCGTGCTTTATTGCACGCACTTTGGGCGGCACGTCGTG
GTGCTGCATTCGTTTGTGAAGAAGGCGCAGGAGGCGCCGCAGAAAGAACTGCGCACCGCCCGAGCGCGTTTGAGCGAGGT
ACGAAATGGTGAAGGCGGTTGCTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12223.94 Da
• Isoelectric Point: 9.5971

>AT58636 WP_080471805.1 NZ_CP013424:204884-205225 [Burkholderia sp. MSMB0852]
MVKAVAKRVRAEGFSPVPHMTDDTERLLAKPGVKAQYDALEDEYSALRAILTARQDAGLTQAQVAERMGTTASAVSRLEA
SLSSERHSPSFATLRKYAAACGKRLVISFAENL

Nucleotide

• Length: 342 bp

>AT58636 NZ_CP013424:204884-205225 [Burkholderia sp. MSMB0852]
ATGGTGAAGGCGGTTGCTAAGCGCGTGCGCGCTGAAGGATTTAGTCCTGTTCCGCATATGACGGATGACACTGAGCGTCT
ACTGGCAAAGCCTGGTGTCAAAGCACAATACGATGCGCTAGAAGATGAATACAGCGCGCTTCGAGCCATCTTGACGGCTC
GGCAAGACGCCGGCTTGACGCAGGCTCAGGTGGCCGAGCGCATGGGAACGACAGCTTCTGCAGTTTCGCGATTGGAAGCG
TCACTGTCGAGCGAAAGGCACTCGCCGTCGTTCGCGACGTTGCGAAAGTATGCGGCAGCTTGTGGTAAGCGATTGGTGAT
CTCGTTTGCCGAGAATTTGTAA

Structures

Toxin

• AlphaFold DB: A0A1B4S475