Detailed information of TA system TA058618

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 222162..222819
• Replicon: chromosome
• Accession: NZ_CP013416
• Organism: Burkholderia ubonensis strain MSMB2035

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A103RX51
• Locus tag: WJ32_RS20555
• Protein ID: WP_059749068.1
• Coordinates: 222487..222819 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WJ32_RS33770
• Protein ID: WP_080409818.1
• Coordinates: 222162..222494 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WJ32_RS20535	217236..218711	+	1476	WP_059749014.1	PLP-dependent aminotransferase family protein	-
WJ32_RS20540	218782..219204	-	423	WP_059749015.1	NUDIX domain-containing protein	-
WJ32_RS20545	219499..219798	+	300	WP_010100493.1	helix-turn-helix domain-containing protein	-
WJ32_RS20550	219809..221161	+	1353	WP_059749016.1	aspartate aminotransferase family protein	-
WJ32_RS34930	221486..222040	+	555	WP_155122471.1	hypothetical protein	-
WJ32_RS33770	222162..222494	-	333	WP_080409818.1	helix-turn-helix domain-containing protein	Antitoxin
WJ32_RS20555	222487..222819	-	333	WP_059749068.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WJ32_RS20560	223139..223612	+	474	WP_059749017.1	cupin domain-containing protein	-
WJ32_RS20565	223626..224369	+	744	WP_080409823.1	SDR family oxidoreductase	-
WJ32_RS20570	224420..224719	-	300	WP_010097871.1	AzlD family protein	-
WJ32_RS20575	224712..225449	-	738	WP_010097873.1	AzlC family ABC transporter permease	-
WJ32_RS20580	225575..226033	+	459	WP_006486309.1	Lrp/AsnC family transcriptional regulator	-
WJ32_RS20585	226137..226706	+	570	WP_059749019.1	hypothetical protein	-
WJ32_RS20590	226751..227383	-	633	WP_059749020.1	LysE family translocator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-105)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12733.78 Da
• Isoelectric Point: 10.4257

>T58618 WP_059749068.1 NZ_CP013416:c222819-222487 [Burkholderia ubonensis]
IKLDWTIVYYNERVKRDVFAFPAGILASYLRLLDLMQEFGADLRMPHSRAMGNGLFELRPTGREGIGRVFYCTHVGRQVV
VLHSFVKKSQETPQQELRIAQARLREVRNG

Nucleotide

• Length: 333 bp

>T58618 NZ_CP013416:c222819-222487 [Burkholderia ubonensis]
ATAAAACTTGATTGGACGATCGTCTACTACAACGAACGCGTGAAGCGTGACGTTTTCGCGTTCCCCGCCGGGATTCTTGC
GAGCTACCTCCGGCTGCTGGACCTGATGCAGGAATTCGGCGCAGATCTACGAATGCCGCACTCCCGCGCAATGGGTAACG
GGCTGTTCGAGTTGCGTCCCACGGGCAGGGAAGGTATCGGGCGAGTGTTCTATTGCACACATGTGGGGCGGCAGGTCGTT
GTGCTGCACTCCTTCGTCAAGAAGTCTCAGGAGACTCCGCAGCAAGAATTGCGAATCGCCCAAGCACGTTTGAGAGAGGT
GCGTAATGGCTAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 11671.25 Da
• Isoelectric Point: 9.9225

>AT58618 WP_080409818.1 NZ_CP013416:c222494-222162 [Burkholderia ubonensis]
MAKGATKRSRADGFNPVLHTADDTARLLADGKVKAAYDALEDEYTALRALLAARQEAGLTQAQVAERMGTTASAVSRLEA
SLTSEKHSPSFATLRKYAAACGKRLVISFA

Nucleotide

• Length: 333 bp

>AT58618 NZ_CP013416:c222494-222162 [Burkholderia ubonensis]
ATGGCTAAGGGAGCAACGAAGCGCAGTCGCGCTGACGGATTCAATCCGGTTCTGCATACGGCTGACGACACGGCCCGTCT
GCTCGCAGATGGCAAGGTCAAGGCCGCCTATGATGCGCTGGAAGATGAATACACCGCGCTGCGCGCACTATTAGCTGCTC
GGCAAGAGGCTGGACTGACTCAAGCACAGGTCGCTGAGCGTATGGGTACGACGGCATCAGCAGTGTCGCGCCTCGAAGCT
TCGCTAACTAGCGAAAAGCACTCGCCCTCGTTCGCGACACTGCGAAAATATGCGGCCGCATGTGGCAAGAGGCTCGTGAT
TTCGTTTGCTTAG

Structures

Toxin

• AlphaFold DB: A0A103RX51