Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 216608..217223 Replicon chromosome
Accession NZ_CP013403
Organism Burkholderia metallica strain FL-6-5-30-S1-D7

Toxin (Protein)


Gene name higB Uniprot ID A0A1B4I0W4
Locus tag WJ16_RS20850 Protein ID WP_069259002.1
Coordinates 216882..217223 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID B1YYE6
Locus tag WJ16_RS20845 Protein ID WP_012365189.1
Coordinates 216608..216895 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WJ16_RS34310 211831..212104 + 274 Protein_198 MFS transporter -
WJ16_RS20815 212119..212946 + 828 WP_069258997.1 SDR family oxidoreductase -
WJ16_RS20820 213035..213814 - 780 WP_069258998.1 5'/3'-nucleotidase SurE -
WJ16_RS20825 213989..214297 - 309 WP_069258999.1 AzlD family protein -
WJ16_RS20830 214290..215024 - 735 WP_069259000.1 AzlC family ABC transporter permease -
WJ16_RS20835 215149..215607 + 459 WP_012365188.1 Lrp/AsnC family transcriptional regulator -
WJ16_RS20840 215827..216396 + 570 WP_069259001.1 hypothetical protein -
WJ16_RS20845 216608..216895 - 288 WP_012365189.1 helix-turn-helix domain-containing protein Antitoxin
WJ16_RS20850 216882..217223 - 342 WP_069259002.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
WJ16_RS20855 217439..218071 - 633 WP_012365191.1 LysE family translocator -
WJ16_RS20860 218502..220139 + 1638 WP_069261061.1 ShlB/FhaC/HecB family hemolysin secretion/activation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(21-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(37-87)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13060.31 Da        Isoelectric Point: 10.4003

>T58595 WP_069259002.1 NZ_CP013403:c217223-216882 [Burkholderia metallica]
MRTVAYEIHFFSEQVEAGVFALPKTLTARFFALADRMEQYGPNLGEPHTRPIGDGLYEMRLKGQEGIARVFYCAVVNRRI
VMLHCFAKKTQKTPQKELKTARRRLKEIRCGNL

Download         Length: 342 bp

>T58595 NZ_CP013403:c217223-216882 [Burkholderia metallica]
ATGCGAACCGTCGCGTATGAAATCCATTTCTTCAGCGAGCAAGTCGAGGCAGGCGTGTTTGCGTTGCCGAAGACGCTGAC
CGCCAGGTTCTTCGCACTCGCTGACCGTATGGAGCAGTACGGCCCGAATCTCGGCGAACCTCACACCAGGCCGATCGGCG
ACGGGCTGTATGAAATGCGCTTGAAGGGCCAGGAAGGTATCGCCCGTGTGTTTTACTGTGCGGTCGTCAACCGGCGGATC
GTCATGCTTCACTGCTTCGCGAAGAAGACGCAGAAAACGCCGCAGAAAGAGCTGAAAACCGCGCGCCGCCGATTGAAGGA
GATTCGTTGTGGCAACCTATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10389.01 Da        Isoelectric Point: 10.6526

>AT58595 WP_012365189.1 NZ_CP013403:c216895-216608 [Burkholderia metallica]
VATYKALRERALSDPKVRAEYDRLNREEFALLDAMLAARRAAGLSQADVAERMGTKAPAVTRLERALATGRHSPSIDTLR
KYAAACGKKLVISFA

Download         Length: 288 bp

>AT58595 NZ_CP013403:c216895-216608 [Burkholderia metallica]
GTGGCAACCTATAAGGCACTGCGCGAGCGCGCGTTGTCCGATCCGAAGGTACGCGCCGAATACGACCGGCTGAACCGCGA
GGAATTCGCGCTGCTCGATGCAATGCTAGCCGCCCGGCGAGCCGCCGGTCTTTCGCAAGCCGATGTGGCGGAGCGCATGG
GCACGAAGGCGCCTGCCGTCACGCGGCTCGAACGCGCGCTCGCGACAGGCCGGCACTCTCCATCGATCGACACGCTGCGC
AAGTATGCCGCCGCGTGCGGCAAGAAACTCGTGATCTCTTTCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B4I0W4

Antitoxin

Source ID Structure
AlphaFold DB B1YYE6

References