Detailed information of TA system TA058575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 228263..228920
• Replicon: chromosome
• Accession: NZ_CP013389
• Organism: Burkholderia sp. BDU8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1B4FXL7
• Locus tag: WS71_RS14510
• Protein ID: WP_066488983.1
• Coordinates: 228263..228595 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WS71_RS14515
• Protein ID: WP_082727864.1
• Coordinates: 228588..228920 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WS71_RS14485	223747..225111	+	1365	WP_066488978.1	type II secretory pathway protein	-
WS71_RS14490	225188..225769	+	582	WP_066488980.1	hypothetical protein	-
WS71_RS14495	226121..226411	+	291	WP_159086292.1	hypothetical protein	-
WS71_RS14500	226488..227267	+	780	WP_066488982.1	hypothetical protein	-
WS71_RS14505	227634..228221	+	588	WP_203234344.1	site-specific integrase	-
WS71_RS14510	228263..228595	+	333	WP_066488983.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WS71_RS14515	228588..228920	+	333	WP_082727864.1	helix-turn-helix domain-containing protein	Antitoxin
WS71_RS35600	229318..230214	+	897	WP_159086293.1	hypothetical protein	-
WS71_RS35605	230217..230651	+	435	WP_159086294.1	hypothetical protein	-
WS71_RS14525	230991..231218	+	228	WP_066489056.1	hypothetical protein	-
WS71_RS14530	231368..231832	+	465	WP_066488986.1	hypothetical protein	-
WS71_RS14535	231829..232119	+	291	WP_066488988.1	type II toxin-antitoxin system RelE/ParE family toxin	-
WS71_RS14540	232657..233229	+	573	WP_066488989.1	SocA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	218341..234473	16132

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-105)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12617.65 Da
• Isoelectric Point: 10.8950

>T58575 WP_066488983.1 NZ_CP013389:228263-228595 [Burkholderia sp. BDU8]
MKQNWAVVYYNERVRRDVFALPAGILADYLRLLDLMQEFGADLRMPHSRAMGGGLFELRPKGREGIGRVFYCTHVGRHVV
VLHSFVKKAHATPQNELRTARARLNEVRNG

Nucleotide

• Length: 333 bp

>T58575 NZ_CP013389:228263-228595 [Burkholderia sp. BDU8]
ATGAAACAGAATTGGGCAGTCGTCTACTACAACGAGCGTGTCAGGCGCGACGTCTTTGCTTTGCCGGCCGGGATTCTGGC
GGACTATCTCCGCCTGCTGGATCTGATGCAGGAGTTCGGCGCGGATCTGCGAATGCCACATTCGCGGGCAATGGGGGGCG
GATTATTTGAGTTGCGCCCGAAGGGTAGGGAAGGTATTGGACGCGTGTTCTACTGCACTCACGTTGGGCGGCACGTCGTC
GTGCTGCATTCGTTCGTGAAGAAGGCACACGCGACGCCGCAGAATGAGCTGAGGACCGCTCGGGCACGTTTGAATGAGGT
ACGGAATGGCTAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 11835.50 Da
• Isoelectric Point: 10.1761

>AT58575 WP_082727864.1 NZ_CP013389:228588-228920 [Burkholderia sp. BDU8]
MAKVVTKRARVEGFNPVPHKADDTERLLSKGGVRAAYDALEDEYAALRAILSARQEAGLTQAQVAERMGTTASAVSRLEA
SLSNEKHSPSFATLRKYAAACGKRLVITFA

Nucleotide

• Length: 333 bp

>AT58575 NZ_CP013389:228588-228920 [Burkholderia sp. BDU8]
ATGGCTAAGGTGGTAACAAAGCGTGCTCGCGTTGAGGGGTTTAACCCAGTTCCGCACAAGGCGGATGATACAGAGCGCTT
GCTTTCGAAGGGAGGTGTGAGGGCTGCGTATGACGCATTGGAAGACGAATATGCTGCGCTGCGAGCCATCCTGTCGGCTA
GGCAGGAGGCCGGTCTAACGCAAGCGCAGGTTGCCGAACGTATGGGCACGACGGCGTCGGCTGTGTCTCGACTTGAGGCG
TCGCTGTCAAATGAAAAACACTCACCGTCATTCGCAACATTGCGAAAGTATGCAGCCGCTTGCGGAAAAAGATTGGTCAT
TACATTCGCTTAA

Structures

Toxin

• AlphaFold DB: A0A1B4FXL7