Detailed information of TA system TA058553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2736902..2737508
• Replicon: chromosome
• Accession: NZ_CP013377
• Organism: Burkholderia pseudomultivorans strain SUB-INT23-BP2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: WS57_RS11805
• Protein ID: WP_059516151.1
• Coordinates: 2736902..2737234 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A132F2X6
• Locus tag: WS57_RS11810
• Protein ID: WP_009691377.1
• Coordinates: 2737221..2737508 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WS57_RS11780	2733552..2733971	-	420	WP_009691384.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
WS57_RS11785	2733982..2734308	-	327	WP_009691383.1	thioredoxin family protein	-
WS57_RS11790	2734507..2734839	+	333	WP_009691382.1	DUF2322 family protein	-
WS57_RS11795	2735004..2735852	+	849	WP_059604418.1	hypothetical protein	-
WS57_RS11800	2736050..2736682	+	633	WP_009691379.1	LysE family translocator	-
WS57_RS11805	2736902..2737234	+	333	WP_059516151.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WS57_RS11810	2737221..2737508	+	288	WP_009691377.1	helix-turn-helix transcriptional regulator	Antitoxin
WS57_RS11815	2737542..2738111	-	570	WP_009691376.1	hypothetical protein	-
WS57_RS11820	2738306..2738764	-	459	WP_009691375.1	Lrp/AsnC family transcriptional regulator	-
WS57_RS11825	2738889..2739629	+	741	WP_009691374.1	AzlC family ABC transporter permease	-
WS57_RS11830	2739622..2739921	+	300	WP_009691373.1	AzlD family protein	-
WS57_RS11835	2740120..2740899	+	780	WP_069244246.1	5'/3'-nucleotidase SurE	-
WS57_RS11840	2741040..2741789	-	750	WP_059516156.1	SDR family oxidoreductase	-
WS57_RS11845	2741786..2742256	-	471	WP_059516158.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-90)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12667.95 Da
• Isoelectric Point: 10.1776

>T58553 WP_059516151.1 NZ_CP013377:2736902-2737234 [Burkholderia pseudomultivorans]
MRFEIRFFSERVEAGIFALPKTLIARFFALADRMERYGPNLGEPHTRAMGDGLYEMRLKGAEGIARVFYCAVVGEQIVML
HCFVKKTAKMPLKELDTARRRLKEVRDGNL

Nucleotide

• Length: 333 bp

>T58553 NZ_CP013377:2736902-2737234 [Burkholderia pseudomultivorans]
CTGCGCTTTGAAATCCGGTTTTTCAGCGAGCGTGTCGAGGCAGGCATTTTTGCCTTGCCGAAGACGTTGATCGCGCGGTT
CTTCGCACTCGCCGACCGGATGGAACGGTACGGCCCGAATCTGGGCGAGCCTCACACCCGGGCGATGGGCGACGGGTTGT
ATGAAATGCGCCTGAAGGGGGCGGAAGGCATCGCCCGTGTGTTTTACTGCGCAGTCGTCGGCGAGCAGATCGTCATGCTT
CACTGCTTCGTGAAAAAAACCGCGAAAATGCCGTTGAAAGAGCTGGATACCGCGCGCCGTCGATTGAAGGAGGTTCGAGA
TGGCAACCTATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10473.10 Da
• Isoelectric Point: 10.2231

>AT58553 WP_009691377.1 NZ_CP013377:2737221-2737508 [Burkholderia pseudomultivorans]
MATYKELRERALADPKVHAEYQRLNREEFALLDAMLAARRAAGLSQADVAERMGTKAPAVTRLERSLATGQHSPSIETLR
KYAAACGKKLVITFA

Nucleotide

• Length: 288 bp

>AT58553 NZ_CP013377:2737221-2737508 [Burkholderia pseudomultivorans]
ATGGCAACCTATAAGGAACTGCGCGAGCGCGCGCTGGCCGATCCGAAGGTACACGCCGAGTATCAGCGGTTGAATCGCGA
GGAATTCGCGCTGCTCGATGCCATGCTTGCCGCGCGGCGAGCCGCCGGTCTGTCGCAAGCCGATGTTGCGGAGCGCATGG
GCACGAAGGCGCCGGCCGTCACGCGACTCGAACGCTCGCTTGCGACAGGCCAGCACTCGCCGTCGATCGAAACGCTGCGC
AAGTATGCGGCCGCCTGCGGCAAGAAACTCGTGATCACTTTCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A132F2X6