Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 726546..727139 Replicon chromosome
Accession NZ_CP013372
Organism Burkholderia sp. NRF60-BP8

Toxin (Protein)


Gene name higB Uniprot ID A0A807EC46
Locus tag WS54_RS03360 Protein ID WP_034205316.1
Coordinates 726861..727139 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A807ECA8
Locus tag WS54_RS03355 Protein ID WP_059784218.1
Coordinates 726546..726848 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WS54_RS03330 721783..723054 + 1272 WP_034205321.1 Rieske 2Fe-2S domain-containing protein -
WS54_RS03335 723069..723545 + 477 WP_034205320.1 aromatic-ring-hydroxylating dioxygenase subunit beta -
WS54_RS03340 723581..723907 + 327 WP_059784224.1 non-heme iron oxygenase ferredoxin subunit -
WS54_RS03345 723897..725117 + 1221 WP_034205319.1 FAD-dependent oxidoreductase -
WS54_RS03350 725226..726512 + 1287 WP_059784221.1 DUF445 domain-containing protein -
WS54_RS03355 726546..726848 - 303 WP_059784218.1 HigA family addiction module antidote protein Antitoxin
WS54_RS03360 726861..727139 - 279 WP_034205316.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
WS54_RS03365 727526..727825 - 300 WP_059784215.1 putative addiction module antidote protein -
WS54_RS03370 727829..728146 - 318 WP_108041857.1 type II toxin-antitoxin system RelE/ParE family toxin -
WS54_RS03380 728542..730182 + 1641 WP_034205314.1 acetolactate synthase large subunit -
WS54_RS03385 730212..731645 + 1434 WP_059784211.1 aldehyde dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(11-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-87)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10711.22 Da        Isoelectric Point: 7.8644

>T58523 WP_034205316.1 NZ_CP013372:c727139-726861 [Burkholderia sp. NRF60-BP8]
MINSFRCKDTQLLFAGETPKRFRAIETVATRKLQMIAAAKELRDLRAPPGNRLEALQGDREGQHSIRINERWRICFEWSD
DGPVQVEIVDYH

Download         Length: 279 bp

>T58523 NZ_CP013372:c727139-726861 [Burkholderia sp. NRF60-BP8]
ATGATCAATTCGTTCCGATGCAAAGACACGCAGTTGCTTTTCGCAGGAGAAACGCCCAAGCGCTTCCGCGCGATCGAAAC
GGTTGCCACCCGGAAACTGCAAATGATTGCCGCAGCGAAGGAGCTGCGCGATCTGCGGGCGCCGCCCGGCAACAGGCTCG
AAGCGTTGCAGGGCGATCGGGAAGGTCAGCACAGCATCCGCATCAACGAGCGATGGCGGATTTGTTTCGAATGGAGCGAC
GACGGCCCCGTCCAGGTCGAGATCGTCGACTATCACTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11595.36 Da        Isoelectric Point: 8.5562

>AT58523 WP_059784218.1 NZ_CP013372:c726848-726546 [Burkholderia sp. NRF60-BP8]
MLKNGMRPIHPGEILREEFLVPLELSVNALALALNVPATRLHEIVKERRGITADTAYRLGRYFDTSPEFWLNLQATYDLK
TLPTRNEIDRKVEPRERTHA

Download         Length: 303 bp

>AT58523 NZ_CP013372:c726848-726546 [Burkholderia sp. NRF60-BP8]
ATGCTCAAGAACGGAATGCGCCCGATTCATCCGGGCGAAATCCTTCGGGAAGAATTCCTCGTTCCGCTGGAATTGAGCGT
CAACGCGCTGGCGCTCGCGTTGAACGTGCCGGCAACGCGGTTGCACGAAATCGTCAAGGAACGGCGCGGAATCACGGCCG
ATACGGCCTATCGGCTCGGGCGCTACTTCGATACGTCGCCGGAATTCTGGCTGAATCTGCAAGCCACGTACGATCTCAAG
ACGCTTCCGACGCGCAACGAGATCGACCGCAAGGTCGAACCGCGCGAACGCACGCACGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A807EC46

Antitoxin

Source ID Structure
AlphaFold DB A0A807ECA8

References