Detailed information of TA system TA058514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 185185..185851
• Replicon: chromosome
• Accession: NZ_CP013371
• Organism: Burkholderia ubonensis strain RF23-BP41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: WI31_RS22555
• Protein ID: WP_059651870.1
• Coordinates: 185519..185851 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WI31_RS35830
• Protein ID: WP_080402075.1
• Coordinates: 185185..185511 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WI31_RS22530	180441..181916	+	1476	WP_059651873.1	PLP-dependent aminotransferase family protein	-
WI31_RS22535	181987..182409	-	423	WP_059652075.1	NUDIX domain-containing protein	-
WI31_RS22540	182595..183320	+	726	WP_059652071.1	class I SAM-dependent methyltransferase	-
WI31_RS22545	183422..183721	+	300	WP_010100493.1	helix-turn-helix domain-containing protein	-
WI31_RS22550	183732..185084	+	1353	WP_059651871.1	aspartate aminotransferase family protein	-
WI31_RS35830	185185..185511	-	327	WP_080402075.1	helix-turn-helix domain-containing protein	Antitoxin
WI31_RS22555	185519..185851	-	333	WP_059651870.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WI31_RS22560	186172..186645	+	474	WP_059651866.1	cupin domain-containing protein	-
WI31_RS22565	186659..187402	+	744	WP_080401501.1	SDR family oxidoreductase	-
WI31_RS22570	187445..187744	-	300	WP_010097871.1	AzlD family protein	-
WI31_RS22575	187737..188474	-	738	WP_059612286.1	AzlC family ABC transporter permease	-
WI31_RS22580	188600..189058	+	459	WP_039322714.1	Lrp/AsnC family transcriptional regulator	-
WI31_RS22585	189163..189732	+	570	WP_059612288.1	hypothetical protein	-
WI31_RS22590	189785..190417	-	633	WP_059613842.1	LysE family translocator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-100)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12749.80 Da
• Isoelectric Point: 10.4257

>T58514 WP_059651870.1 NZ_CP013371:c185851-185519 [Burkholderia ubonensis]
MKPDWTIVYYNERVKRDVFAFPAGILASYLRLLDLMQEFGADLRMPHSRAMGNGLFELRPTGREGIGRVFYCTHVGRQVV
VLHSFVKKTQETPQQELRIAQARLREVRNG

Nucleotide

• Length: 333 bp

>T58514 NZ_CP013371:c185851-185519 [Burkholderia ubonensis]
ATGAAACCTGATTGGACGATCGTCTACTACAACGAGCGCGTGAAGCGTGACGTTTTCGCGTTCCCCGCCGGGATTCTTGC
GAGCTACCTCCGGCTGCTAGACCTGATGCAGGAATTCGGCGCAGATCTACGAATGCCGCACTCCCGCGCAATGGGTAACG
GGCTGTTCGAGTTGCGTCCCACGGGCAGGGAAGGTATCGGGCGAGTGTTCTATTGCACACATGTGGGGCGGCAGGTCGTT
GTGCTGCACTCCTTCGTCAAGAAGACTCAGGAGACGCCGCAGCAAGAATTGCGAATCGCCCAAGCACGTTTGAGAGAGGT
GCGTAATGGCTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11526.04 Da
• Isoelectric Point: 8.9662

>AT58514 WP_080402075.1 NZ_CP013371:c185511-185185 [Burkholderia ubonensis]
MKRSRADGFNPVPHTADDTARLLADGKVKAAYDALEDEYTALRALLAARQEAGLTQAQVAERMGTTASAVSRLEASLTSE
KHSPSFATLRKYAAACGKRLVISFAEAV

Nucleotide

• Length: 327 bp

>AT58514 NZ_CP013371:c185511-185185 [Burkholderia ubonensis]
ATGAAGCGTAGTCGCGCTGACGGATTCAATCCGGTTCCGCATACGGCTGACGACACGGCCCGTCTGCTCGCAGACGGCAA
GGTCAAGGCGGCCTATGATGCGCTGGAAGATGAATACACCGCGCTGCGCGCACTATTGGCTGCTCGGCAAGAGGCTGGAT
TGACTCAAGCACAGGTCGCTGAGCGTATGGGTACGACGGCGTCAGCCGTGTCGCGCCTCGAAGCATCGCTCACCAGCGAA
AAACATTCGCCCTCGTTTGCGACATTGCGAAAATATGCAGCCGCATGTGGCAAGAGGCTCGTGATTTCGTTTGCTGAGGC
TGTGTGA