Detailed information of TA system TA058495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 1037824..1038479
• Replicon: chromosome
• Accession: NZ_CP013358
• Organism: Burkholderia oklahomensis C6786

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U4UL57
• Locus tag: WI23_RS34530
• Protein ID: WP_010121214.1
• Coordinates: 1038135..1038479 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WI23_RS34525
• Protein ID: WP_010121216.1
• Coordinates: 1037824..1038138 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WI23_RS05160	1033960..1035153	-	1194	WP_010105766.1	pyridoxal phosphate-dependent aminotransferase	-
WI23_RS05165	1035214..1035651	-	438	WP_010105764.1	transcription antitermination factor NusB	-
WI23_RS05170	1035648..1036166	-	519	WP_010105762.1	6,7-dimethyl-8-ribityllumazine synthase	-
WI23_RS05175	1036247..1037383	-	1137	WP_010105760.1	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II	-
WI23_RS34525	1037824..1038138	-	315	WP_010121216.1	transcriptional regulator	Antitoxin
WI23_RS34530	1038135..1038479	-	345	WP_010121214.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WI23_RS05190	1038577..1039140	+	564	WP_107950779.1	hypothetical protein	-
WI23_RS05195	1039294..1039923	-	630	WP_010105743.1	riboflavin synthase	-
WI23_RS05200	1039938..1041074	-	1137	WP_045568144.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-
WI23_RS05205	1041103..1042386	-	1284	WP_010116635.1	glutamate-1-semialdehyde 2,1-aminomutase	-
WI23_RS05210	1042681..1043139	-	459	WP_010116633.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (44-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-90)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13394.28 Da
• Isoelectric Point: 9.1937

>T58495 WP_010121214.1 NZ_CP013358:c1038479-1038135 [Burkholderia oklahomensis C6786]
MEATFIELPTFSRERENYLADDEYAVLQKELLANPEAGDLIKGAGGLRKLRFADKRRQKGKRGGLRVIYYYWVSGYQFWL
FTLYDKDEMADLSSDERKAFAKLLEREVTIRSKQ

Nucleotide

• Length: 345 bp

>T58495 NZ_CP013358:c1038479-1038135 [Burkholderia oklahomensis C6786]
ATGGAAGCGACCTTCATTGAACTGCCGACGTTTTCCCGTGAACGCGAGAACTACCTGGCGGATGACGAGTACGCCGTCCT
GCAGAAAGAGCTGCTGGCCAATCCCGAGGCTGGCGACCTGATCAAGGGAGCCGGCGGTCTGCGGAAGTTGAGGTTCGCCG
ACAAACGCCGCCAGAAAGGCAAACGCGGTGGCCTACGGGTGATCTATTACTACTGGGTCAGCGGTTATCAATTCTGGTTG
TTCACACTGTACGACAAGGATGAAATGGCAGACCTCTCCAGTGACGAGCGCAAAGCCTTCGCAAAGCTACTGGAAAGAGA
GGTCACGATACGGAGCAAGCAATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11590.43 Da
• Isoelectric Point: 10.3388

>AT58495 WP_010121216.1 NZ_CP013358:c1038138-1037824 [Burkholderia oklahomensis C6786]
MKKRNLFAELTEGLDALTAEREGKITLRKIKADVPDALEVSPAEIKAVREASHASQAVMARSLRINVRTYQNWEQGKAKP
NAQAATLIRLVAKHPETLQMLETL

Nucleotide

• Length: 315 bp

>AT58495 NZ_CP013358:c1038138-1037824 [Burkholderia oklahomensis C6786]
ATGAAGAAGCGAAATCTGTTTGCTGAATTGACCGAGGGTCTCGATGCCCTTACTGCCGAGCGCGAAGGGAAAATCACGCT
GCGCAAGATCAAGGCCGATGTGCCCGATGCGCTCGAAGTTTCGCCAGCAGAAATCAAGGCCGTGCGTGAAGCCTCTCACG
CGTCGCAAGCCGTCATGGCCCGAAGCCTGCGCATCAATGTGCGTACGTATCAGAATTGGGAACAAGGCAAGGCCAAGCCC
AACGCACAAGCGGCCACGCTGATCCGACTGGTCGCCAAGCACCCGGAAACGCTTCAGATGCTGGAAACGCTCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4UL57