Detailed information of TA system TA058494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2352117..2352712
• Replicon: chromosome
• Accession: NZ_CP013357
• Organism: Burkholderia oklahomensis EO147

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: WG70_RS25590
• Protein ID: WP_010111175.1
• Coordinates: 2352117..2352395 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WG70_RS25595
• Protein ID: WP_010111178.1
• Coordinates: 2352413..2352712 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WG70_RS25555	2347328..2347657	-	330	WP_159086819.1	IS66 family insertion sequence element accessory protein TnpB	-
WG70_RS25560	2347675..2348115	-	441	WP_081464416.1	hypothetical protein	-
WG70_RS25565	2348233..2349075	+	843	WP_038802590.1	IS3 family transposase	-
WG70_RS25575	2349862..2350524	-	663	WP_010111169.1	DUF2612 domain-containing protein	-
WG70_RS25580	2350524..2351705	-	1182	WP_010111171.1	baseplate J/gp47 family protein	-
WG70_RS25585	2351702..2352055	-	354	WP_010111173.1	hypothetical protein	-
WG70_RS25590	2352117..2352395	+	279	WP_010111175.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WG70_RS25595	2352413..2352712	+	300	WP_010111178.1	HigA family addiction module antidote protein	Antitoxin
WG70_RS25600	2352778..2353516	-	739	Protein_2071	hypothetical protein	-
WG70_RS25605	2353520..2353897	+	378	WP_010111182.1	hypothetical protein	-
WG70_RS25610	2353894..2354862	-	969	WP_010111184.1	hypothetical protein	-
WG70_RS25615	2354906..2355316	-	411	WP_010111186.1	hypothetical protein	-
WG70_RS25620	2355303..2355647	-	345	WP_144444658.1	hypothetical protein	-
WG70_RS35350	2355647..2356066	-	420	WP_144444657.1	hypothetical protein	-
WG70_RS25625	2356129..2356443	-	315	WP_010111189.1	hypothetical protein	-
WG70_RS25630	2356443..2357018	-	576	WP_010111191.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2335770..2425219	89449

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10677.16 Da
• Isoelectric Point: 10.2114

>T58494 WP_010111175.1 NZ_CP013357:2352117-2352395 [Burkholderia oklahomensis EO147]
MITSFNCRDSEALFNGRRVARFANIERVAIRKLQQLHAAADLDFLRIPPNNRLEALKGDRQGQFSIRINDQWRICFRFAG
GRASDVEIVDYH

Nucleotide

• Length: 279 bp

>T58494 NZ_CP013357:2352117-2352395 [Burkholderia oklahomensis EO147]
ATGATTACATCGTTCAACTGCCGAGATTCGGAAGCGCTATTCAACGGCCGGCGGGTTGCTCGCTTTGCCAACATCGAGCG
CGTAGCTATCCGCAAGCTCCAGCAGTTGCATGCTGCGGCCGATCTAGACTTCCTCCGCATCCCGCCGAACAACCGGCTCG
AAGCACTGAAGGGAGACCGGCAAGGCCAATTCAGCATCCGCATCAACGATCAGTGGCGCATTTGCTTCAGGTTCGCTGGA
GGCCGCGCCTCTGATGTGGAAATCGTCGACTACCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10953.55 Da
• Isoelectric Point: 5.6088

>AT58494 WP_010111178.1 NZ_CP013357:2352413-2352712 [Burkholderia oklahomensis EO147]
MTREVPLATPGEILALDWLEPMGISQYALAKAISVPARRINEIVKGDRAITADTAVRLGAFFGVDPQSWMNLQTHYDTAL
AKEKIGDDVLREIREHAHA

Nucleotide

• Length: 300 bp

>AT58494 NZ_CP013357:2352413-2352712 [Burkholderia oklahomensis EO147]
ATGACCCGAGAAGTACCCCTGGCGACGCCCGGTGAAATCCTGGCGCTCGACTGGCTGGAGCCGATGGGCATCAGCCAATA
CGCCCTCGCAAAAGCGATCAGTGTTCCGGCCCGCCGCATCAACGAAATCGTCAAAGGCGACCGTGCCATTACTGCAGATA
CGGCCGTGCGCCTCGGCGCGTTCTTCGGCGTGGATCCGCAGAGCTGGATGAATCTCCAGACGCACTACGACACCGCACTC
GCCAAGGAGAAAATTGGCGATGACGTGCTGCGCGAGATCCGAGAGCACGCGCACGCCTGA