Detailed information of TA system TA058475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 39939..40666
• Replicon: plasmid pKPYL1
• Accession: NZ_CP013339
• Organism: Raoultella ornithinolytica strain Yangling I2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A663AUB1
• Locus tag: ATN83_RS25645
• Protein ID: WP_000558568.1
• Coordinates: 40355..40666 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: ATN83_RS25640
• Protein ID: WP_000990392.1
• Coordinates: 39939..40358 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATN83_RS25610	35626..35988	-	363	WP_000783215.1	arsenite efflux transporter metallochaperone ArsD	-
ATN83_RS25615	36036..36389	-	354	WP_001114073.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
ATN83_RS28780	36705..37298	+	594	WP_044348674.1	hypothetical protein	-
ATN83_RS25625	37722..38381	+	660	WP_000078540.1	hypothetical protein	-
ATN83_RS25630	38378..38707	+	330	WP_004118613.1	hypothetical protein	-
ATN83_RS25635	38700..39902	+	1203	WP_064357855.1	hypothetical protein	-
ATN83_RS25640	39939..40358	-	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
ATN83_RS25645	40355..40666	-	312	WP_000558568.1	type II toxin-antitoxin system HigB family toxin	Toxin
ATN83_RS25650	40877..41257	+	381	Protein_44	gluconate permease	-
ATN83_RS25655	41307..42002	-	696	WP_000370412.1	lactate utilization protein C	-
ATN83_RS25660	41995..43422	-	1428	WP_000023895.1	iron-sulfur cluster-binding protein	-
ATN83_RS25665	43433..44152	-	720	WP_001102107.1	(Fe-S)-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..152010	152010

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12372.11 Da
• Isoelectric Point: 10.0935

>T58475 WP_000558568.1 NZ_CP013339:c40666-40355 [Raoultella ornithinolytica]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T58475 NZ_CP013339:c40666-40355 [Raoultella ornithinolytica]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT58475 WP_000990392.1 NZ_CP013339:c40358-39939 [Raoultella ornithinolytica]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT58475 NZ_CP013339:c40358-39939 [Raoultella ornithinolytica]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A663AUB1

Antitoxin

• AlphaFold DB: V0AHC4