Detailed information of TA system TA058454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 52092..52749
• Replicon: plasmid pCAV1193-166
• Accession: NZ_CP013324
• Organism: Klebsiella pneumoniae strain CAV1193

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: AGG09_RS28345
• Protein ID: WP_000270043.1
• Coordinates: 52092..52442 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AGG09_RS28350
• Protein ID: WP_000124640.1
• Coordinates: 52447..52749 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AGG09_RS28305	48566..49063	-	498	WP_000062185.1	hypothetical protein	-
AGG09_RS28310	49066..49554	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
AGG09_RS28315	49651..49986	+	336	WP_000683477.1	hypothetical protein	-
AGG09_RS28320	50001..50471	-	471	WP_001281821.1	hypothetical protein	-
AGG09_RS28325	50464..50835	-	372	WP_000516918.1	hypothetical protein	-
AGG09_RS28330	50846..51040	-	195	WP_000343597.1	hypothetical protein	-
AGG09_RS28340	51381..51929	-	549	WP_001061573.1	hypothetical protein	-
AGG09_RS28345	52092..52442	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AGG09_RS28350	52447..52749	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
AGG09_RS28355	52776..53069	-	294	WP_001239998.1	hypothetical protein	-
AGG09_RS28360	53158..53430	-	273	WP_001043046.1	HU family DNA-binding protein	-
AGG09_RS28365	53488..54015	-	528	WP_004201083.1	thermonuclease family protein	-
AGG09_RS34180	54018..54323	-	306	WP_171820680.1	hypothetical protein	-
AGG09_RS28375	54235..55092	-	858	WP_001167036.1	hypothetical protein	-
AGG09_RS28380	55079..55309	-	231	WP_000972665.1	hypothetical protein	-
AGG09_RS28385	55309..55827	-	519	WP_000210757.1	nitrite reductase	-
AGG09_RS28390	55824..56270	-	447	WP_000919343.1	hypothetical protein	-
AGG09_RS28395	56270..56629	-	360	WP_000422769.1	hypothetical protein	-
AGG09_RS28400	56687..57115	-	429	WP_000591076.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-30 / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / sul1 / qnrA1 / qacE / ant(2'')-Ia	-	1..166486	166486

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T58454 WP_000270043.1 NZ_CP013324:52092-52442 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T58454 NZ_CP013324:52092-52442 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT58454 WP_000124640.1 NZ_CP013324:52447-52749 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT58454 NZ_CP013324:52447-52749 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9