Detailed information of TA system TA058419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 356736..357351
• Replicon: chromosome
• Accession: NZ_CP013320
• Organism: Vibrio cholerae strain NCTC9420

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: ASZ86_RS16015
• Protein ID: WP_001162670.1
• Coordinates: 356736..357023 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASZ86_RS16020
• Protein ID: WP_001232701.1
• Coordinates: 357034..357351 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ86_RS15970	352228..352485	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ86_RS15975	352473..352775	+	303	WP_000229318.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ86_RS15980	353064..353288	+	225	WP_001894423.1	DUF3709 domain-containing protein	-
ASZ86_RS15985	353732..353851	+	120	WP_071919600.1	DUF645 family protein	-
ASZ86_RS20405	353855..353908	+	54	Protein_332	DUF645 family protein	-
ASZ86_RS16000	354510..355514	+	1005	WP_000964931.1	LD-carboxypeptidase	-
ASZ86_RS16010	356289..356469	+	181	Protein_334	DUF645 family protein	-
ASZ86_RS16015	356736..357023	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ86_RS16020	357034..357351	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
ASZ86_RS20775	357348..357458	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
ASZ86_RS16030	357504..358043	+	540	WP_000099372.1	hypothetical protein	-
ASZ86_RS16035	358188..358622	+	435	WP_000453680.1	GNAT family N-acetyltransferase	-
ASZ86_RS16040	358849..359127	+	279	WP_001921601.1	DUF3709 domain-containing protein	-
ASZ86_RS20425	359379..359585	+	207	Protein_341	DUF3709 domain-containing protein	-
ASZ86_RS20430	359785..359886	+	102	WP_001921603.1	hypothetical protein	-
ASZ86_RS16045	359876..360262	+	387	WP_000703163.1	VOC family protein	-
ASZ86_RS16050	360457..360708	+	252	WP_001890111.1	TIGR03643 family protein	-
ASZ86_RS16055	360681..360830	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
ASZ86_RS16060	360944..361387	+	444	WP_000803902.1	hypothetical protein	-
ASZ86_RS16065	361391..361501	+	111	WP_071908336.1	DUF3265 domain-containing protein	-
ASZ86_RS16075	361714..361866	+	153	WP_001884520.1	DUF645 family protein	-
ASZ86_RS16080	362067..362303	+	237	Protein_349	nucleotide pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304702..436238	131536
	-	inside	Integron	-	-	309782..435862	126080

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T58419 WP_001162670.1 NZ_CP013320:356736-357023 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T58419 NZ_CP013320:356736-357023 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT58419 WP_001232701.1 NZ_CP013320:357034-357351 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT58419 NZ_CP013320:357034-357351 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36