Detailed information of TA system TA058335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 767054..767669
• Replicon: chromosome
• Accession: NZ_CP013312
• Organism: Vibrio cholerae strain E1320

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: ASZ88_RS17840
• Protein ID: WP_001162670.1
• Coordinates: 767054..767341 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASZ88_RS17845
• Protein ID: WP_001232701.1
• Coordinates: 767352..767669 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ88_RS17810	763261..763710	+	450	WP_001894449.1	hypothetical protein	-
ASZ88_RS17825	765309..766316	+	1008	WP_000093800.1	DUF4365 domain-containing protein	-
ASZ88_RS20745	766313..766423	+	111	Protein_733	DUF3265 domain-containing protein	-
ASZ88_RS17835	766607..766787	+	181	Protein_734	DUF645 family protein	-
ASZ88_RS17840	767054..767341	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ88_RS17845	767352..767669	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
ASZ88_RS21165	767666..767776	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
ASZ88_RS21170	767953..768078	+	126	WP_001944767.1	DUF645 family protein	-
ASZ88_RS21175	768094..768132	+	39	WP_106019119.1	hypothetical protein	-
ASZ88_RS17865	768356..769096	+	741	WP_000469836.1	PhzF family phenazine biosynthesis protein	-
ASZ88_RS17870	769301..770131	+	831	WP_000056616.1	abortive infection family protein	-
ASZ88_RS17875	770188..770670	+	483	WP_001895003.1	hypothetical protein	-
ASZ88_RS17885	771117..771992	+	876	WP_000259044.1	hypothetical protein	-
ASZ88_RS17890	772122..772577	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	688711..843534	154823
	-	inside	Integron	catB9	-	693791..843158	149367

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T58335 WP_001162670.1 NZ_CP013312:767054-767341 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T58335 NZ_CP013312:767054-767341 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT58335 WP_001232701.1 NZ_CP013312:767352-767669 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT58335 NZ_CP013312:767352-767669 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36