Detailed information of TA system TA058333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 739223..739838
• Replicon: chromosome
• Accession: NZ_CP013312
• Organism: Vibrio cholerae strain E1320

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: ASZ88_RS17550
• Protein ID: WP_001162670.1
• Coordinates: 739223..739510 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASZ88_RS17555
• Protein ID: WP_001232701.1
• Coordinates: 739521..739838 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ88_RS17505	734715..734972	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ88_RS17510	734960..735262	+	303	WP_000229318.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ88_RS17515	735551..735775	+	225	WP_001894423.1	DUF3709 domain-containing protein	-
ASZ88_RS17520	736219..736338	+	120	WP_071919600.1	DUF645 family protein	-
ASZ88_RS20665	736342..736395	+	54	Protein_681	DUF645 family protein	-
ASZ88_RS17535	736997..738001	+	1005	WP_000964931.1	LD-carboxypeptidase	-
ASZ88_RS17545	738776..738956	+	181	Protein_683	DUF645 family protein	-
ASZ88_RS17550	739223..739510	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ88_RS17555	739521..739838	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
ASZ88_RS21145	739835..739945	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
ASZ88_RS17565	739991..740530	+	540	WP_000099372.1	hypothetical protein	-
ASZ88_RS17570	740675..741109	+	435	WP_000453680.1	GNAT family N-acetyltransferase	-
ASZ88_RS17575	741336..741614	+	279	WP_001921601.1	DUF3709 domain-containing protein	-
ASZ88_RS20685	741866..742072	+	207	Protein_690	DUF3709 domain-containing protein	-
ASZ88_RS20260	742272..742373	+	102	WP_001921603.1	hypothetical protein	-
ASZ88_RS17580	742363..742749	+	387	WP_000703163.1	VOC family protein	-
ASZ88_RS17585	742944..743195	+	252	WP_001890111.1	TIGR03643 family protein	-
ASZ88_RS17590	743168..743317	+	150	WP_071908333.1	DUF3265 domain-containing protein	-
ASZ88_RS17595	743530..743682	+	153	WP_001884520.1	DUF645 family protein	-
ASZ88_RS17600	743883..744254	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
ASZ88_RS17605	744550..744732	+	183	WP_001900194.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	688711..843534	154823
	-	inside	Integron	catB9	-	693791..843158	149367

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T58333 WP_001162670.1 NZ_CP013312:739223-739510 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T58333 NZ_CP013312:739223-739510 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGACGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT58333 WP_001232701.1 NZ_CP013312:739521-739838 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT58333 NZ_CP013312:739521-739838 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36